FastQCFastQC Report
Thu 26 May 2016
SRR1512754_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512754_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336107
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35940.2689904326524747No Hit
GTACATGGGAAGCAGTGGTATCAAC25240.1889070261588331No Hit
GGTATCAACGCAGAGTACTTTTTTT22710.16997141695986923No Hit
TATCAACGCAGAGTACTTTTTTTTT19990.14961376596335474No Hit
CCCATGTACTCTGCGTTGATACCAC16980.12708563011794716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC1001.2171222E-712.5115821
GTATTAA1603.6379788E-1212.0303671
CTAGGTC907.5091866E-611.6040013
ATCTAGT751.8374552E-411.5491531
GTCCTAA2950.011.41865351
GTCTTTA1552.4920155E-1011.1765991
GACCGTG700.001498793710.8504947
GATATAC1354.6946298E-810.693661
CCCTTAG750.002376381810.2659141
CCAATAC1303.8704638E-710.2245033
CTAGTAC750.002660622610.1271283
GTTAATC856.6288223E-410.0526643
GTCTAAA1158.788476E-610.0427411
CAATAGT951.6542317E-49.9934994
GGCGAGG3050.09.96073319
GTTCTAG1451.336357E-79.9561661
TCAGTAC1054.130695E-59.9462863
TCTAGAC1451.6298145E-79.8215683
GTCCTAG1803.3378456E-99.6242941
GCTCTAG909.884139E-49.6242941
Produced by FastQC (version 0.11.5)