FastQCFastQC Report
Thu 26 May 2016
SRR1512754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336107
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43500.32557272733396353No Hit
GTACATGGGAAGCAGTGGTATCAAC28690.21472831143014742No Hit
GGTATCAACGCAGAGTACTTTTTTT28230.2112854733939722No Hit
TATCAACGCAGAGTACTTTTTTTTT26620.19923554026735885No Hit
CCCATGTACTCTGCGTTGATACCAC20540.15373020274573818No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG802.8062559E-511.8993241
GTATAAG1451.1750672E-911.1607471
CTAATAC803.740564E-410.6965453
TGCTTAA803.7756987E-410.6857310
TTAACCT1002.3753912E-510.4588454
TCTATAC1659.1495167E-1010.3724093
AGGACGT5050.010.1673965
GTTTATA1605.9608283E-910.1144261
TAGAACA2550.010.0673374
ATTATAC1909.822543E-1110.0084643
GGACGTG5150.09.9598886
GTCCTAT1356.259161E-79.8720331
ATAGGAC1356.3627886E-79.860193
TAATACT1651.0066287E-89.79616454
TTATACT1752.5320332E-99.7796994
ACTGTTC1951.6370905E-109.7419768
TAGGACT2350.09.710344
CGTGAAA2550.09.6846719
GGGTAAG800.0044391649.519461
GCATTAC800.0044391649.519461
Produced by FastQC (version 0.11.5)