FastQCFastQC Report
Thu 26 May 2016
SRR1512753_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512753_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022116
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46920.23203416619026804No Hit
GGTATCAACGCAGAGTACTTTTTTT29070.14376029861788345No Hit
TATCAACGCAGAGTACTTTTTTTTT26530.13119919925464218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCT400.005295214214.2437454
ACTGCGT751.543813E-512.6140078
TAGGACC3800.011.9947324
CGAGCGA658.096965E-411.67990810
TAGGACT3100.011.3337334
ATAGGAC2400.011.0792973
AACCGTC600.00589918111.0776427
AAGGCGT1303.2909156E-810.95618256
GTTAGAC803.784428E-410.6836063
GTATAAA3200.010.526251
AGGACGT11250.010.2127855
GGTATCA14650.010.1823891
GCCCTAT1053.5602454E-510.0822871
GTCCTAC10900.010.0654691
GTAGGAC11450.09.8697663
TGTAGGA11750.09.8602362
TCCTACA12500.09.8004292
GGACGTG11400.09.7452356
TAGGACG11850.09.6961644
CTAGGAC2950.09.6574973
Produced by FastQC (version 0.11.5)