FastQCFastQC Report
Thu 26 May 2016
SRR1512753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022116
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56350.2786684839049787No Hit
GGTATCAACGCAGAGTACTTTTTTT35950.17778406382225354No Hit
TATCAACGCAGAGTACTTTTTTTTT33540.16586585537130413No Hit
GTCCTACAGTGGACATTTCTAAATT21950.10854965788312837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4050.010.798314
GGTATCA14850.010.6402351
CTTAGAC1553.61797E-910.427263
ACTAGAC1201.5093174E-610.2994753
GTCTTAC1502.3725079E-810.1530911
TGAACCG750.002631009610.1412735
GTTCTAA2550.010.0784361
GTCTAGG1803.8016879E-1010.047331
GGACGTG10400.010.0464946
GACGTGG5300.010.0366517
AGACCCG951.6314333E-410.0078345
TTAGACA2105.456968E-129.9599314
GTCCTAC9950.09.9490091
GTCCTAA6900.09.9323721
GAATTAC1151.0031314E-59.9323721
AGGACGT10750.09.9054295
GTCTTAG2500.09.8992631
GTATAAA2700.09.8710611
TAGACAG3200.09.804555
ATAAGAC1651.0095391E-89.7953053
Produced by FastQC (version 0.11.5)