FastQCFastQC Report
Thu 26 May 2016
SRR1512752_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512752_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083908
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48300.23177606688970911No Hit
GTCCTACAGTGGACATTTCTAAATT41370.19852123990118567No Hit
CTGTAGGACGTGGAATATGGCAAGA36930.17721511698213166No Hit
GTCCTAAAGTGTGTATTTCTCATTT36780.1764953155321636No Hit
GTACATGGGAAGCAGTGGTATCAAC35230.16905736721582718No Hit
CTTTAGGACGTGAAATATGGCGAGG31530.15130226478328218No Hit
GGTATCAACGCAGAGTACTTTTTTT29120.13973745482046232No Hit
TATCAACGCAGAGTACTTTTTTTTT26950.12932432717759132No Hit
GTCCTACAGTGTGCATTTCTCATTT23980.11507225846822412No Hit
CCCATGTACTCTGCGTTGATACCAC22500.10797021749520612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG2650.012.3491771
TAGGACC7300.012.2266744
TCCAACG2200.011.21778918
GGCGAGG12150.011.01697319
GTGTAGG4600.010.8805421
CCAACGA1408.447387E-910.84954219
TAGAAAT6200.010.7203674
AGGACCG803.791225E-410.6815655
TTAGGAC15400.010.6062913
TAGGACA7250.010.4774444
GACGTGA13100.010.4374537
ACGTGAA14650.010.39757258
TAAGACC1752.382876E-1010.3090564
TTTAGGA16450.010.2756632
TTTAGAA6950.010.2478372
AGGACGT23100.010.2345735
TTTAGGC1303.8629878E-710.2268173
ATAGGAC2600.010.2268173
GGACGTG22700.010.2062766
ATTTAGA6050.010.1819191
Produced by FastQC (version 0.11.5)