FastQCFastQC Report
Thu 26 May 2016
SRR1512752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512752_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083908
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59810.2870088314839235No Hit
GTCCTACAGTGGACATTTCTAAATT46490.22309046272676142No Hit
CTGTAGGACGTGGAATATGGCAAGA41310.19823331932119845No Hit
GTCCTAAAGTGTGTATTTCTCATTT39140.18782019167832745No Hit
GTACATGGGAAGCAGTGGTATCAAC38640.18542085351176732No Hit
GGTATCAACGCAGAGTACTTTTTTT37460.1797584154386854No Hit
CTTTAGGACGTGAAATATGGCGAGG36380.17457584499891549No Hit
TATCAACGCAGAGTACTTTTTTTTT36200.17371208325895385No Hit
CCCATGTACTCTGCGTTGATACCAC27310.13105185065751462No Hit
GTCCTACAGTGTGCATTTCTCATTT25560.12265416707455416No Hit
CTGTAGGACCTGGAATATGGCGAGA21690.1040832896653787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1900.013.99283918
TAGGACC8250.012.4467284
CGGTTTC1459.822543E-1111.7879213
CCAACGT1052.7395072E-711.75588819
CCGGTTT1657.2759576E-1211.51147112
GCCGGTT1604.5474735E-1111.27764411
TGTAGGA24300.010.8403282
TTACACC2400.010.6964084
AGGACGT23550.010.5374375
GGACGTG23450.010.4896876
TACACCG1105.987422E-610.3722745
GATTTAC1201.484872E-610.3126351
GTCCTAC21600.010.2244931
CATTTCT28550.010.17717214
GGCGAGG11350.010.12257219
TAGAAAT6200.010.1213314
TAGGACG25050.010.0962324
GTGTAGG3400.010.0793181
GACGTGG11750.010.0225687
CTGTAGG25100.010.012391
Produced by FastQC (version 0.11.5)