FastQCFastQC Report
Thu 26 May 2016
SRR1512751_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512751_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594734
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35750.2241753170121161No Hit
GTCCTACAGTGGACATTTCTAAATT30090.18868350458446362No Hit
GTACATGGGAAGCAGTGGTATCAAC29840.18711584502493833No Hit
CTGTAGGACGTGGAATATGGCAAGA26110.16372636439682103No Hit
GTCCTAAAGTGTGTATTTCTCATTT25830.1619705856901527No Hit
CTTTAGGACGTGAAATATGGCGAGG21490.13475601573679372No Hit
CCCATGTACTCTGCGTTGATACCAC21420.13431707106012664No Hit
GGTATCAACGCAGAGTACTTTTTTT21410.13425436467774562No Hit
TATCAACGCAGAGTACTTTTTTTTT18400.11537974358106116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6050.012.5567254
AGTCGAT550.003094171412.07746110
ATCCTAC1603.6379788E-1111.4251021
TCCAACG752.0868318E-411.3923518
ATTAGAC600.005892698711.0790433
TGGACCG951.3669351E-510.9950475
TGTAGGA17700.010.7303082
GTCCTAT1751.8735591E-1010.4458071
GTCCTAA8600.010.292381
AGGACCT10350.010.2755655
CCTACAC2600.010.2268093
GGACCTG9850.010.12202556
GTAGGAC17550.010.1185893
ATTTAGG2600.09.9911821
GGCGAGG6850.09.97996119
TTAGGAC10850.09.9777033
TCTTATG1252.581859E-69.8761762
GTCTTAG2157.2759576E-129.8448861
GGACGTG16400.09.8428166
CTGTAGG15700.09.8049821
Produced by FastQC (version 0.11.5)