FastQCFastQC Report
Thu 26 May 2016
SRR1512751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594734
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41930.26292786132358126No Hit
GTACATGGGAAGCAGTGGTATCAAC33750.2116340405359138No Hit
GTCCTACAGTGGACATTTCTAAATT31960.2004095980897128No Hit
CTGTAGGACGTGGAATATGGCAAGA29590.18554818546541305No Hit
GTCCTAAAGTGTGTATTTCTCATTT27650.17338314728349682No Hit
GGTATCAACGCAGAGTACTTTTTTT26680.16730062819253871No Hit
CCCATGTACTCTGCGTTGATACCAC25030.1569540750996718No Hit
TATCAACGCAGAGTACTTTTTTTTT24140.15137320706776178No Hit
CTTTAGGACGTGAAATATGGCGAGG23810.14930389644918837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG604.0585577E-412.6790415
TAGGACC5550.012.5065244
GGCGAGG6750.011.81484719
GGCTAAC657.901713E-411.7136631
TTAGGAC8950.010.9422673
TCCAACG1053.4917994E-610.85036918
ACCTTTT4650.010.82154115
TCGAACT1002.4150537E-510.443480519
GACGTGA8100.010.2014977
GTTCCCG750.0026523910.1311429
AGGACCT10800.010.1256245
GTCCTAA8600.010.0706861
CACCTTT5300.010.03241714
AGGACGT15400.010.0032695
CTACACA3350.09.9341344
GTCTAGA1356.2814615E-79.8698461
TCTATAC1856.384653E-109.7650613
TGGCGAG16800.09.6636118
GTCCTAC15500.09.5787531
TGTAGGA16700.09.5662222
Produced by FastQC (version 0.11.5)