FastQCFastQC Report
Thu 26 May 2016
SRR1512750_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512750_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936804
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44910.2318768445335718No Hit
GTATCAACGCAGAGTACTTTTTTTT42890.22144729151736572No Hit
CTGTAGGACGTGGAATATGGCAAGA39810.20554480474018022No Hit
GTCCTAAAGTGTGTATTTCTCATTT38000.19619951218605497No Hit
CTTTAGGACGTGAAATATGGCGAGG31570.16300048946615145No Hit
GGTATCAACGCAGAGTACTTTTTTT26140.1349646118037757No Hit
GTCCTACAGTGTGCATTTCTCATTT25100.12959494094394683No Hit
TATCAACGCAGAGTACTTTTTTTTT23750.12262469511628435No Hit
GTACATGGGAAGCAGTGGTATCAAC22280.11503487188171856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2000.013.76275818
TAGGACC8950.013.4758184
AACCGTG752.0814333E-411.3957897
CCAACGA1604.5474735E-1111.27210219
TGTAGGA25300.011.1864622
GTCCTAC23350.010.9659141
GTAGGAC24500.010.9315073
AGGACCT15600.010.835745
AGGACGT21900.010.6672935
GGCGAGG11000.010.61411419
GTATTAC1105.1656734E-610.5011921
CTGTAGG23450.010.4676071
GGACCTG15650.010.4367676
GTGTAGG5100.010.3810821
GGACGTG22200.010.3517616
TTAGGAC13800.010.3230783
ACCGTGC1106.355878E-610.3203778
TCCTACA25650.010.2932952
GACGTGA12150.010.2390157
GTCCTAG2550.010.1923351
Produced by FastQC (version 0.11.5)