FastQCFastQC Report
Thu 26 May 2016
SRR1512750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936804
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51830.2676058083316639No Hit
GTCCTACAGTGGACATTTCTAAATT47800.24679833374982701No Hit
CTGTAGGACGTGGAATATGGCAAGA45710.236007360579594No Hit
GTCCTAAAGTGTGTATTTCTCATTT40730.21029489819310576No Hit
CTTTAGGACGTGAAATATGGCGAGG36700.18948742361126888No Hit
GGTATCAACGCAGAGTACTTTTTTT32700.16883484338115784No Hit
TATCAACGCAGAGTACTTTTTTTTT30320.15654655814424176No Hit
GTCCTACAGTGTGCATTTCTCATTT27090.13986959960842707No Hit
GTACATGGGAAGCAGTGGTATCAAC25090.12954330949337156No Hit
CTGTAGGACCTGGAATATGGCGAGA20890.10785810025175495No Hit
CTGAAGGACCTGGAATATGGCGAGA20560.1061542623827708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7900.013.8408394
AACCGCT550.00307141512.0895947
GTCCTAG2950.011.6126611
CTAATAC1501.7462298E-1011.4096673
TGTAGGA26700.011.3957182
GTAGGAC25850.011.29196553
AGGACGT24100.011.165355
GGACGTG24000.011.0815556
CTGTAGG26000.011.0531211
AGGACCT14800.010.9859445
GGACCTG14300.010.8933576
CTAGTAC1408.24366E-910.8663493
TAGGACG25750.010.7081014
TAATACT2500.010.6490234
GACGTGG13250.010.5384767
TTAGGAC13450.010.3917043
GACGTGA12700.010.3217127
TAGGACA8050.010.1576744
TCCAACG1701.5770638E-910.05201618
ATAGGAC2850.010.008483
Produced by FastQC (version 0.11.5)