FastQCFastQC Report
Thu 26 May 2016
SRR1512749_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512749_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740896
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT42840.2460801794018712No Hit
GTATCAACGCAGAGTACTTTTTTTT41870.2405083359373564No Hit
GTCCTAAAGTGTGTATTTCTCATTT38660.2220695549877764No Hit
CTGTAGGACGTGGAATATGGCAAGA36400.20908773413230888No Hit
CTTTAGGACGTGAAATATGGCGAGG34120.19599102990643902No Hit
GGTATCAACGCAGAGTACTTTTTTT26040.1495781482638825No Hit
TATCAACGCAGAGTACTTTTTTTTT24130.13860678639045643No Hit
GTCCTACAGTGTGCATTTCTCATTT20820.11959358858886458No Hit
GTACATGGGAAGCAGTGGTATCAAC19170.11011571053066926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAC400.00529506114.2436483
TCCAACG1051.3915269E-913.55832518
CCAACGA957.476774E-812.98819819
TAGGACC6450.012.5134374
GTATTAA2150.011.6349261
GGCGAGG9900.011.12120819
GGACCTG12750.010.7987946
ACACCGT803.7877183E-410.6824276
GTCCTAC21150.010.4627581
CGAAATC1201.5277983E-610.28945913
AGGACCT13500.010.1988615
GTAGGAC22150.010.16025453
TAGCACA2450.010.0768474
TGTAGGA23300.010.0663272
AGGACGT25050.010.045135
TCCTACA23800.010.0144422
GACGTGG11700.09.9824397
GGACGTG24950.09.9331596
CACCTTT6600.09.78832614
GTCCTAA13500.09.7637251
Produced by FastQC (version 0.11.5)