FastQCFastQC Report
Thu 26 May 2016
SRR1512749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740896
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52310.3004774552873922No Hit
GTCCTACAGTGGACATTTCTAAATT45760.2628531514806169No Hit
CTGTAGGACGTGGAATATGGCAAGA42630.24487390401264636No Hit
GTCCTAAAGTGTGTATTTCTCATTT40520.23275370843519658No Hit
CTTTAGGACGTGAAATATGGCGAGG39090.22453954745142732No Hit
GGTATCAACGCAGAGTACTTTTTTT33210.19076383655313126No Hit
TATCAACGCAGAGTACTTTTTTTTT30490.17513969817840927No Hit
GTCCTACAGTGTGCATTTCTCATTT23070.13251796775913094No Hit
GTACATGGGAAGCAGTGGTATCAAC20430.11735336286601843No Hit
CTGAAGGACCTGGAATATGGCGAGA20360.11695127106961013No Hit
CTGTAGGACCTGGAATATGGCGAGA17910.10287805819532012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1055.456968E-1215.37111518
TAGGACC7900.013.1172984
AGGACCG907.39355E-611.620715
CCGGTTT907.4768486E-611.60902112
AGGACCT14800.011.5636055
GTACTAG752.0445189E-411.4176361
AATGTCC6450.011.3400218
AAATGTC6250.011.2469447
GCCGGTT951.3617571E-510.99896811
GGCGAGG11400.010.99296619
GGTCTAA1158.64311E-710.7557451
TAGAAAT7700.010.7417134
ACTGTTC4350.010.7001468
TTAGGAC16200.010.6807413
GGACCTG14900.010.58296
AGGACGT25700.010.5807245
TGTCCAC7200.010.55456510
TCTAGGA3250.010.5326852
TGGCGAG24950.010.42619318
GTATAAG2100.010.4208571
Produced by FastQC (version 0.11.5)