FastQCFastQC Report
Thu 26 May 2016
SRR1512748_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512748_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027929
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT43950.21672356379340696No Hit
GTATCAACGCAGAGTACTTTTTTTT43770.21583595875398007No Hit
GTCCTAAAGTGTGTATTTCTCATTT41500.20464227297898496No Hit
GTACATGGGAAGCAGTGGTATCAAC40020.1973441870992525No Hit
CTGTAGGACGTGGAATATGGCAAGA39710.19581553397579501No Hit
CTTTAGGACGTGAAATATGGCGAGG34100.1681518435803226No Hit
GGTATCAACGCAGAGTACTTTTTTT27490.1355570140769228No Hit
CCCATGTACTCTGCGTTGATACCAC26240.12939309019201362No Hit
TATCAACGCAGAGTACTTTTTTTTT23840.11755835633298799No Hit
GTCCTACAGTGTGCATTTCTCATTT22930.1130710197447741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG802.0089155E-613.0574654
TAGGACC5750.012.22137454
GCCGGTT1157.174094E-811.55387311
GTATACG600.00532491611.2310471
TGTCGAG1105.022539E-711.21820818
TTTGCCG600.005899018611.07768519
TCCAACG2150.011.03759318
CCAACGA1408.421921E-910.8516119
GTCCTAA12400.010.7911211
ACACCGT803.7886546E-410.6823196
CCGGTTT1252.269735E-710.63009212
GGCCCGT909.564261E-510.5504396
TAGGACA7300.010.5370324
ACTGTTC4950.010.5099938
TTAGGAC13650.010.5058883
CTACACT2450.010.4653524
GTCGAGG1002.412873E-510.444674519
AGGCGTG1002.412873E-510.44467457
TAATCTT1856.002665E-1110.2663115
TAAGACT1951.4551915E-1110.2268264
Produced by FastQC (version 0.11.5)