FastQCFastQC Report
Thu 26 May 2016
SRR1512748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027929
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51640.2546440235333683No Hit
GTCCTACAGTGGACATTTCTAAATT47410.23378530510683562No Hit
CTGTAGGACGTGGAATATGGCAAGA45940.22653653061818238No Hit
GTCCTAAAGTGTGTATTTCTCATTT43000.21203898164087598No Hit
GTACATGGGAAGCAGTGGTATCAAC42610.2101158373887843No Hit
CTTTAGGACGTGAAATATGGCGAGG39730.19591415675795357No Hit
GGTATCAACGCAGAGTACTTTTTTT33090.16317139308131595No Hit
TATCAACGCAGAGTACTTTTTTTTT31800.15681022363208968No Hit
CCCATGTACTCTGCGTTGATACCAC30250.1491669580148023No Hit
GTCCTACAGTGTGCATTTCTCATTT25920.12781512567747688No Hit
CTGAAGGACCTGGAATATGGCGAGA22810.11247928305182284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTG500.001501951713.2974957
TGATCGT550.003073330312.08863310
GTACTAG802.8218064E-511.89359951
TAGGACC7500.011.6629374
GACGTGG12700.011.218367
GTCGAGG1053.4926397E-610.85054819
GGCGAGG10400.010.77229819
AGGACGT25550.010.6438675
GGACGTG25100.010.63371856
TGTAGGA25600.010.6230772
AAATGTC6100.010.5881697
TCCAACG1553.6998244E-910.41302618
GGTTTAG1105.935095E-610.37986851
GTCCTAC22200.010.3720761
CTGTAGG26150.010.3335591
CGTGGAA13800.010.3241429
CTAGGAC3550.010.1773953
ACGTGGA13950.010.1450438
GACTGCG951.6475105E-49.99811657
TAGGACT3050.09.9754284
Produced by FastQC (version 0.11.5)