FastQCFastQC Report
Thu 26 May 2016
SRR1512747_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512747_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321630
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33550.2538531964316791No Hit
GTACATGGGAAGCAGTGGTATCAAC25030.18938734744217367No Hit
GTCCTACAGTGGACATTTCTAAATT23960.18129128424748228No Hit
GTCCTAAAGTGTGTATTTCTCATTT22360.1691850215264484No Hit
CTGTAGGACGTGGAATATGGCAAGA21290.161088958331757No Hit
GGTATCAACGCAGAGTACTTTTTTT20580.1557168042492982No Hit
CTTTAGGACGTGAAATATGGCGAGG20350.15397652898314962No Hit
CCCATGTACTCTGCGTTGATACCAC19340.14633445064049697No Hit
TATCAACGCAGAGTACTTTTTTTTT18290.13838971572981848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG852.7129136E-713.405663519
TAGGACC3250.011.9777954
TTCTGCG658.0655166E-411.6847647
GGCGAGG6300.011.60582819
TTACACC907.505394E-611.6045034
GTCCTAT1709.094947E-1211.319661
TTAGGAC8900.011.30859853
GTACTAG600.005343450711.2253281
CAAGGGT1105.0020026E-711.2208834
CTACACT2300.011.1458274
AGGACGT13500.011.0418615
TTTAGGA9350.010.9674352
GACGTGA6900.010.8697957
CATAACC700.001498233110.8509654
CCGTCCA700.00151737410.8344649
GGACGTG13650.010.7805866
CTTTAGG8500.010.7536761
GTCTTAA1907.2759576E-1210.6345231
TAGGACG14600.010.5350974
TAGGACA4200.010.398844
Produced by FastQC (version 0.11.5)