##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512747_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1321630 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.1664981878438 31.0 26.0 34.0 10.0 34.0 2 28.68445177545909 31.0 28.0 34.0 16.0 34.0 3 29.83816877643516 31.0 30.0 34.0 25.0 34.0 4 33.68712120638908 37.0 35.0 37.0 28.0 37.0 5 34.050765342796396 37.0 35.0 37.0 30.0 37.0 6 34.32919425255177 37.0 35.0 37.0 31.0 37.0 7 34.32232243517475 37.0 35.0 37.0 32.0 37.0 8 34.36686061908401 37.0 35.0 37.0 32.0 37.0 9 35.98624350234181 39.0 37.0 39.0 32.0 39.0 10 35.93179709903679 39.0 37.0 39.0 32.0 39.0 11 35.96504089646876 39.0 37.0 39.0 32.0 39.0 12 35.88542027647677 39.0 37.0 39.0 31.0 39.0 13 35.87536148543843 39.0 37.0 39.0 31.0 39.0 14 37.12022502515833 40.0 38.0 41.0 31.0 41.0 15 37.02810241898262 40.0 38.0 41.0 31.0 41.0 16 36.93575736022942 40.0 38.0 41.0 31.0 41.0 17 36.90333451873823 40.0 38.0 41.0 31.0 41.0 18 36.833065230056825 40.0 38.0 41.0 31.0 41.0 19 36.858512594296435 40.0 38.0 41.0 31.0 41.0 20 36.79061537646694 40.0 38.0 41.0 30.0 41.0 21 36.706719732451596 40.0 38.0 41.0 30.0 41.0 22 36.59341646300402 40.0 38.0 41.0 30.0 41.0 23 36.46091039095662 40.0 38.0 41.0 30.0 41.0 24 36.36030961766909 40.0 37.0 41.0 30.0 41.0 25 36.258954472885755 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.661983704811963 1101 2 2.1921997699820253 1101 3 1.1505248117876832 1101 4 0.8790150962300629 1101 5 0.42935864019503356 1101 6 0.2895570957500695 1101 7 0.008384632534053083 1101 8 -0.08072016573142804 1101 9 -0.10379513035450572 1101 10 -0.1255847081657535 1101 11 -0.14545271709366148 1101 12 -0.13277386845862083 1101 13 -0.06699577153870706 1101 14 -0.15479328927210645 1101 15 -0.24231644060463964 1101 16 -0.3854065254331829 1101 17 -0.48667223581430363 1101 18 -0.4013332464548327 1101 19 -0.262907893722641 1101 20 -0.3843448992352094 1101 21 -0.5199289311940944 1101 22 -0.24503411842826495 1101 23 -0.5299861583993817 1101 24 -0.4080318133347234 1101 25 -0.4983243018373642 1102 1 3.6578261938600924 1102 2 2.1682281258722433 1102 3 1.129559507864606 1102 4 0.8759149828624828 1102 5 0.49792223100806154 1102 6 0.2784190812222249 1102 7 0.18487765552947621 1102 8 0.11109077633913955 1102 9 -0.13526233073520189 1102 10 -0.11470212058579676 1102 11 -0.08791377323160532 1102 12 -0.022497307555042312 1102 13 -0.14305701802221193 1102 14 -0.1961437630366092 1102 15 -0.3577757192509452 1102 16 -0.3399818168998223 1102 17 -0.47989047539778085 1102 18 -0.46030747982408826 1102 19 -0.3230710870493496 1102 20 -0.472375522476959 1102 21 -0.36258593129206673 1102 22 -0.45601817698752 1102 23 -0.5008863809357322 1102 24 -0.3967503398361458 1102 25 -0.4460450537023277 1103 1 2.5441979247718898 1103 2 1.326845353996756 1103 3 0.21612290568490522 1103 4 -0.08760102983264773 1103 5 -0.4434333671694546 1103 6 -0.5596685499963954 1103 7 -0.6024888174036747 1103 8 -0.6136383441036628 1103 9 -0.9431924336522854 1103 10 -0.8321477665942965 1103 11 -0.7043794512681316 1103 12 -0.8479847852290803 1103 13 -0.9378329405428758 1103 14 -1.1897935600253433 1103 15 -1.2060679974357384 1103 16 -1.4698734123124808 1103 17 -1.4211689055495071 1103 18 -1.4695874295923232 1103 19 -1.6508999877281667 1103 20 -1.6784695540248364 1103 21 -1.4623938686500964 1103 22 -1.76874127172875 1103 23 -1.8000246053588214 1103 24 -1.9467143280907777 1103 25 -1.8707778676574733 1104 1 -6.936572145089016 1104 2 -8.122086128014423 1104 3 -7.734973246389831 1104 4 -5.9035265050662 1104 5 -3.8855072325963036 1104 6 -2.891101837919333 1104 7 -2.2215541550485725 1104 8 -1.7485193478512286 1104 9 -1.9649663965256465 1104 10 -1.6876857343739218 1104 11 -1.6637161362444033 1104 12 -1.7737206033677069 1104 13 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2315 24 -0.2007978844906262 2315 25 0.03018959341382299 2316 1 1.0944152229494648 2316 2 0.9568849876146963 2316 3 0.3605477113591853 2316 4 0.2407415851359147 2316 5 0.0625942455590831 2316 6 -0.187146059880277 2316 7 -0.14220173528163826 2316 8 -0.2949196348777292 2316 9 -0.2474483059352366 2316 10 -0.3183340445986218 2316 11 -0.3282034413793511 2316 12 -0.3699917440029239 2316 13 -0.3202442145413258 2316 14 -0.2749665973927762 2316 15 -0.2451988374264502 2316 16 -0.2892396014354759 2316 17 -0.22625293078160524 2316 18 -0.12696428578311014 2316 19 -0.13534166712096862 2316 20 -0.22652695082863517 2316 21 -0.17352954184933367 2316 22 -0.19645406534289833 2316 23 -0.107236648484907 2316 24 -0.1119099945536064 2316 25 -0.2042365548429288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40190.0 3 1.0 4 387.0 5 1536.0 6 1755.0 7 1680.0 8 1488.0 9 1483.0 10 1500.0 11 1499.0 12 1492.0 13 1592.0 14 1514.0 15 1564.0 16 1622.0 17 1687.0 18 2004.0 19 2392.0 20 2996.0 21 3391.0 22 4023.0 23 4872.0 24 5705.0 25 6530.0 26 7344.0 27 8681.0 28 10007.0 29 12356.0 30 15615.0 31 20399.0 32 27814.0 33 38809.0 34 57145.0 35 86304.0 36 156971.0 37 222003.0 38 565279.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21192091235307 21.02711349805947 12.020422734271083 23.740542855316374 2 14.38625865437307 20.554411168676218 41.6297924468442 23.429537730106517 3 18.482908820482855 25.176185796620842 29.97446472679534 26.36644065610096 4 12.076360904309814 17.337537660603026 38.87006807367438 31.716033361412777 5 12.891638319801215 37.98426843836473 36.11291994723764 13.011173294596412 6 31.04640556129718 37.29429668289065 18.720167663889708 12.939130091922454 7 27.408191558695876 31.81037295266168 22.760523491964264 18.020911996678173 8 24.716822986395822 36.85787862812845 21.198396474367485 17.226901911108243 9 26.579702100101766 15.213794212169882 20.61063260883083 37.59587107889752 10 15.031956754133907 28.969044026778473 34.959324321250676 21.03967489783695 11 33.42268318046015 22.59413400808396 25.310278342219018 18.67290446923688 12 23.448857012615754 25.004342282607126 31.905575642563015 19.6412250622141 13 28.608274403482238 22.326461007681488 26.021556112192524 23.04370847664375 14 20.73972513352186 22.662181920544725 28.057838353380898 28.540254592552515 15 22.957305831055628 30.965760272488474 23.768764944479713 22.30816895197619 16 21.590055474839204 28.325046482724716 28.56075176055936 21.52414628187672 17 21.644314966430592 28.696021443020285 28.14807373549651 21.51158985505261 18 21.324339499521983 27.564304900071324 30.3372687679257 20.774086832480993 19 23.246354519819004 27.021832683343423 28.19841302346183 21.53339977337575 20 23.359272746023777 27.390169513632223 28.599757036021455 20.650800704322545 21 22.63917885802352 26.94584684062284 27.802746756352537 22.6122275450011 22 22.922881340486157 28.324483435299552 27.46245375067589 21.2901814735384 23 21.730004477701577 28.131825358995354 28.319219830820426 21.818950332482643 24 22.805352423024562 27.24054696581881 28.762857713413176 21.19124289774345 25 22.42197829905743 27.381206439956348 28.362206913102817 21.83460834788341 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1356.0 1 1356.0 2 1173.5 3 991.0 4 991.0 5 991.0 6 1569.0 7 2147.0 8 2147.0 9 2147.0 10 2764.5 11 3382.0 12 3382.0 13 3382.0 14 5206.5 15 7031.0 16 7031.0 17 7031.0 18 12238.5 19 17446.0 20 17446.0 21 17446.0 22 27391.0 23 37336.0 24 37336.0 25 37336.0 26 52952.0 27 68568.0 28 68568.0 29 68568.0 30 87402.0 31 106236.0 32 106236.0 33 106236.0 34 119008.5 35 131781.0 36 131781.0 37 131781.0 38 140605.5 39 149430.0 40 149430.0 41 149430.0 42 156100.5 43 162771.0 44 162771.0 45 162771.0 46 168854.0 47 174937.0 48 174937.0 49 174937.0 50 165500.0 51 156063.0 52 156063.0 53 156063.0 54 136855.0 55 117647.0 56 117647.0 57 117647.0 58 100310.5 59 82974.0 60 82974.0 61 82974.0 62 67804.0 63 52634.0 64 52634.0 65 52634.0 66 40339.0 67 28044.0 68 28044.0 69 28044.0 70 20363.0 71 12682.0 72 12682.0 73 12682.0 74 8940.5 75 5199.0 76 5199.0 77 5199.0 78 3553.5 79 1908.0 80 1908.0 81 1908.0 82 1272.0 83 636.0 84 636.0 85 636.0 86 439.0 87 242.0 88 242.0 89 242.0 90 169.0 91 96.0 92 96.0 93 96.0 94 71.5 95 47.0 96 47.0 97 47.0 98 46.5 99 46.0 100 46.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7609315769163838 2 0.3093150125224155 3 0.2909286260148453 4 0.29811671950545915 5 0.30447250743400195 6 0.21193526176009925 7 0.5062687741652354 8 0.24855670649122674 9 0.22184726436294575 10 0.20126661773718818 11 0.24341154483478733 12 0.24204959027867104 13 0.25695542625394396 14 0.2785197067257856 15 0.4800889810309996 16 0.5826895575917617 17 0.5777713883613417 18 0.27920068400384374 19 0.2381907190363415 20 0.22063663809084236 21 0.33594879050869003 22 0.3672737452993652 23 0.47085795570621125 24 0.34949267192784667 25 0.37173792967774644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1321630.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.80865525726475 #Duplication Level Percentage of deduplicated Percentage of total 1 86.25479383188961 65.38859929888166 2 9.918978237433315 15.038888034117873 3 2.1701881337780953 4.935571322309711 4 0.7113309171676372 2.157001610935814 5 0.31160967593415895 1.181135524886032 6 0.16734799865933045 0.7611856043015031 7 0.09083404197792681 0.48202046017499967 8 0.0667250790727883 0.404667081315419 9 0.04931605649839374 0.336472553316107 >10 0.22274958947562437 2.9807844429827726 >50 0.0167863845251913 0.8841036068412522 >100 0.015613116680740387 2.3633056718513226 >500 0.002719656662018699 1.433440448374224 >1k 0.0010072802451921107 1.6528243397112765 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3355 0.2538531964316791 No Hit GTACATGGGAAGCAGTGGTATCAAC 2503 0.18938734744217367 No Hit GTCCTACAGTGGACATTTCTAAATT 2396 0.18129128424748228 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2236 0.1691850215264484 No Hit CTGTAGGACGTGGAATATGGCAAGA 2129 0.161088958331757 No Hit GGTATCAACGCAGAGTACTTTTTTT 2058 0.1557168042492982 No Hit CTTTAGGACGTGAAATATGGCGAGG 2035 0.15397652898314962 No Hit CCCATGTACTCTGCGTTGATACCAC 1934 0.14633445064049697 No Hit TATCAACGCAGAGTACTTTTTTTTT 1829 0.13838971572981848 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.566414200646172E-5 7.566414200646172E-5 10 0.0 0.0 0.0 7.566414200646172E-5 7.566414200646172E-5 11 0.0 0.0 0.0 1.5132828401292345E-4 7.566414200646172E-5 12 0.0 0.0 0.0 1.5132828401292345E-4 7.566414200646172E-5 13 0.0 0.0 0.0 1.5132828401292345E-4 7.566414200646172E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCCG 85 2.7129136E-7 13.4056635 19 TAGGACC 325 0.0 11.977795 4 TTCTGCG 65 8.0655166E-4 11.684764 7 GGCGAGG 630 0.0 11.605828 19 TTACACC 90 7.505394E-6 11.604503 4 GTCCTAT 170 9.094947E-12 11.31966 1 TTAGGAC 890 0.0 11.3085985 3 GTACTAG 60 0.0053434507 11.225328 1 CAAGGGT 110 5.0020026E-7 11.220883 4 CTACACT 230 0.0 11.145827 4 AGGACGT 1350 0.0 11.041861 5 TTTAGGA 935 0.0 10.967435 2 GACGTGA 690 0.0 10.869795 7 CATAACC 70 0.0014982331 10.850965 4 CCGTCCA 70 0.001517374 10.834464 9 GGACGTG 1365 0.0 10.780586 6 CTTTAGG 850 0.0 10.753676 1 GTCTTAA 190 7.2759576E-12 10.634523 1 TAGGACG 1460 0.0 10.535097 4 TAGGACA 420 0.0 10.39884 4 >>END_MODULE