FastQCFastQC Report
Thu 26 May 2016
SRR1512747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512747_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321630
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39730.3006136361916724No Hit
GTACATGGGAAGCAGTGGTATCAAC29190.22086363051686178No Hit
GGTATCAACGCAGAGTACTTTTTTT26480.20035864803311065No Hit
GTCCTACAGTGGACATTTCTAAATT25970.19649977679078107No Hit
GTCCTAAAGTGTGTATTTCTCATTT24920.18855504188010258No Hit
CTGTAGGACGTGGAATATGGCAAGA24690.18681476661395396No Hit
TATCAACGCAGAGTACTTTTTTTTT24220.1832585519396503No Hit
CTTTAGGACGTGAAATATGGCGAGG23170.1753138170289718No Hit
CCCATGTACTCTGCGTTGATACCAC22120.16736908211829332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA250.00603695718.9970847
TCGACTT250.00605678518.98630319
TAGGACC3000.012.6781634
ACCGTGC701.0922243E-412.2124128
GGCGAGG6450.012.06881319
CGAGACG951.0484619E-611.99134918
ACACCGT802.8701004E-511.8731786
CCTATAC1001.9086E-611.4099143
ATTGGAC855.2743115E-511.186193
TGGACCG600.005833680311.0938135
GTCCTAC12300.011.0652241
TGGCGAG13200.011.00342618
TAATACT1408.223651E-910.8669974
CGTGCGC700.001493054910.85547710
CTAGTAG803.7397025E-410.69679453
ACGTGGA6350.010.6204188
AGGACGT14050.010.4903355
CTAGGAC2001.8189894E-1210.4590883
CCTACAG12650.010.4478133
CGTGGAA6400.010.389039
Produced by FastQC (version 0.11.5)