FastQCFastQC Report
Thu 26 May 2016
SRR1512746_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512746_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802445
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47090.2612562380544205No Hit
GTCCTACAGTGGACATTTCTAAATT41940.23268393765135692No Hit
GTCCTAAAGTGTGTATTTCTCATTT36360.20172598886512488No Hit
CTGTAGGACGTGGAATATGGCAAGA36170.2006718651609342No Hit
CTTTAGGACGTGAAATATGGCGAGG31500.174762614115826No Hit
GGTATCAACGCAGAGTACTTTTTTT27290.15140545203875846No Hit
TATCAACGCAGAGTACTTTTTTTTT25790.1430834227951477No Hit
GTCCTACAGTGTGCATTTCTCATTT24140.13392919062717587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTG400.005294624714.2438647
CATTTCG550.003067102312.09178114
GGACGTA550.003079315612.0853666
GGCGAGG11050.011.85550419
TAGGACC7650.011.7913184
GTCTAGG2050.011.7310491
TAGACAG2300.011.5592695
GGCGAAA855.3638323E-511.16822819
GCGCCAC600.00588532411.08103713
AGGACGT24000.011.0380715
TTAGGAC15200.010.9955713
GTCCTAG2750.010.8437551
GGACGTG23350.010.8173086
GACGTGA13300.010.6382757
TAGGACT2500.010.6345274
TGTAGGA22950.010.5513062
CACCTTT5900.010.46685614
GTAGGAC22900.010.4084713
GTCCTAA12500.010.3120611
TTTAGGA16950.010.3085322
Produced by FastQC (version 0.11.5)