FastQCFastQC Report
Thu 26 May 2016
SRR1512746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512746_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802445
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55740.3092466066925759No Hit
GTCCTACAGTGGACATTTCTAAATT42570.23617918993367343No Hit
CTGTAGGACGTGGAATATGGCAAGA41630.23096405160767733No Hit
GTCCTAAAGTGTGTATTTCTCATTT39360.2183700473523464No Hit
CTTTAGGACGTGAAATATGGCGAGG37900.21026993888856524No Hit
GGTATCAACGCAGAGTACTTTTTTT34910.19368136059630114No Hit
TATCAACGCAGAGTACTTTTTTTTT33520.18596961349722185No Hit
GTCCTACAGTGTGCATTTCTCATTT25900.14369370493967917No Hit
CTGTAGGACCTGGAATATGGCGAGA20490.11367891946772302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG1155.3769327E-912.38321818
TAGGACC7550.011.9627674
CCAATAC1209.908035E-911.8837343
GTCGAGG1052.7437454E-711.75422819
GACGTGG12300.011.2753197
TGTAGGA23850.011.0024072
GGACGTG22650.010.9881936
AATGTCC5450.010.98057658
TATGTCG1303.2980097E-810.95438416
AGGACGT23100.010.9074965
GTAGGAC23900.010.7798893
ACGTGGA13350.010.6019598
CTGTAGG23750.010.45581151
GGCGAGG9100.010.43274719
AAATGTC5800.010.3179567
CTAGGAC2500.010.2675463
CGTGGAA13450.010.2406339
TTTAGAA7450.010.2094132
TCCAACG1409.780888E-810.17192818
GTGTTAT1701.5188562E-910.0740591
Produced by FastQC (version 0.11.5)