FastQCFastQC Report
Thu 26 May 2016
SRR1512745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427386
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40080.28079300203308705No Hit
GTCCTACAGTGGACATTTCTAAATT31560.22110347166078415No Hit
GTCCTAAAGTGTGTATTTCTCATTT29320.2054104495910707No Hit
CTGTAGGACGTGGAATATGGCAAGA26200.1835523117082555No Hit
GTACATGGGAAGCAGTGGTATCAAC25700.1800494049962659No Hit
CTTTAGGACGTGAAATATGGCGAGG25050.1754956262706794No Hit
GGTATCAACGCAGAGTACTTTTTTT23400.16393603412111368No Hit
TATCAACGCAGAGTACTTTTTTTTT22000.15412789532754279No Hit
CCCATGTACTCTGCGTTGATACCAC17420.12204126984571798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCC250.006051569718.989257
TCCAACG953.092282E-1014.98623418
TAGGACC4600.013.6217634
GCCGGTT707.3093615E-613.56279611
CCGGTTT751.486455E-512.65994612
GGCGAGG7150.011.94875119
CCAACGA802.8888355E-511.86577319
AGGACGT18900.011.6041355
GTAGGAC17000.011.3947553
TGTAGGA17600.011.38396552
GACGTGG9200.011.352277
GGACGTG18600.011.2302036
AGGACCT10350.011.1913395
TAGGACG20050.011.0328774
GTCTTAT1052.9678686E-610.99828151
CGGTTTC951.36253875E-510.99804113
GTCTTAA1951.8189894E-1210.8572781
AGGCGTG700.001498319610.8510017
GTCTAGG1604.3473847E-1010.8264311
TTAGGAC11700.010.7941773
Produced by FastQC (version 0.11.5)