FastQCFastQC Report
Thu 26 May 2016
SRR1512745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427386
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48240.3379604395727575No Hit
GTCCTACAGTGGACATTTCTAAATT33010.231261901125554No Hit
CTGTAGGACGTGGAATATGGCAAGA30850.21612934412975887No Hit
GTCCTAAAGTGTGTATTTCTCATTT30710.2151485302504018No Hit
GGTATCAACGCAGAGTACTTTTTTT29560.2070918448128257No Hit
CTTTAGGACGTGAAATATGGCGAGG28870.20225783355028001No Hit
TATCAACGCAGAGTACTTTTTTTTT28570.20015608952308625No Hit
GTACATGGGAAGCAGTGGTATCAAC27990.1960927177371783No Hit
CCCATGTACTCTGCGTTGATACCAC20110.14088690795622208No Hit
CTGAAGGACCTGGAATATGGCGAGA15390.10781946859504017No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14610.10235493412433638No Hit
GTCCTACAGTGTGCATTTCTCATTT14320.1003232482313824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1450.013.75088218
TAGGACC3950.012.7562034
CCAACGA1155.3660187E-912.384335519
CGTGCCC658.0322777E-411.69064710
GGCGAGG7350.011.10939419
TGTCCGC600.00588147511.081758510
TGGCGAA600.00589991311.07709918
CCAATAG1053.4345921E-610.8651223
CACCTTT5050.010.34252714
TTAGGAC10300.010.3376883
TGGCGAG16750.010.31658318
GTACTAG1201.4991274E-610.3042911
AGGACGT15500.010.1827425
GGACGTG15350.010.1487516
TAGGCTG1502.4108886E-810.1418485
GACGTGA8600.010.051257
AGGACCT10700.010.0411415
GCACTGC1901.0004442E-109.998936
CCCGTTT1356.453811E-79.85010712
ATGGCGA18500.09.75126917
Produced by FastQC (version 0.11.5)