FastQCFastQC Report
Thu 26 May 2016
SRR1512744_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512744_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746180
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41250.2362299419303852No Hit
GGTATCAACGCAGAGTACTTTTTTT24000.1374428753049514No Hit
TATCAACGCAGAGTACTTTTTTTTT22050.12627564168642408No Hit
GTCCTACAGTGGACATTTCTAAATT19420.11121419326758981No Hit
CTGTAGGACGTGGAATATGGCAAGA18430.10554467466126058No Hit
GTCCTAAAGTGTGTATTTCTCATTT18270.10462838882589423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC1701.8189894E-1211.7298813
TAGGACC4500.011.6047534
GTCCTAT1950.011.3488011
TAGGACA4550.010.4338444
TCCTATA2550.010.4265622
GTATAGG2350.010.2359681
GCCCTAA855.864557E-410.1877981
GTTTAAT1803.1286618E-1010.1563541
GTAATAA1803.1286618E-1010.1563541
TGTAGGA13650.010.0869212
AGGACGT11800.010.0580485
CTACTAG1451.3425961E-79.9535951
GGGTTAG1451.3425961E-79.9535951
CTAGGAC2300.09.9084723
GGTATCA12850.09.8838821
GTAGGAC12450.09.8388353
GTCCTAC12000.09.7821721
TTACACA2750.09.667434
GGACGTG12300.09.6494636
GTCTTAC1908.3491614E-109.621811
Produced by FastQC (version 0.11.5)