FastQCFastQC Report
Thu 26 May 2016
SRR1512744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746180
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49940.2859957163637196No Hit
GGTATCAACGCAGAGTACTTTTTTT31590.18090918462014227No Hit
TATCAACGCAGAGTACTTTTTTTTT29470.16876839730153823No Hit
CTGTAGGACGTGGAATATGGCAAGA22200.12713465965708No Hit
GTCCTACAGTGGACATTTCTAAATT21600.12369858777445625No Hit
GTCCTAAAGTGTGTATTTCTCATTT21150.1211215338624884No Hit
CTTTAGGACGTGAAATATGGCGAGG19450.11138599686172101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGT704.4472654E-714.9254888
TGTCCGA604.0996124E-412.6640510
TAGGACC4700.012.5415424
CTAGGGT1353.6743586E-1011.9721534
AGGACCT8550.010.8975675
GTACCGT700.001493777310.8552116
GTCCTAC11200.010.7929771
TGTAGGA14000.010.7308962
TGCGTGG1002.4043118E-510.44784210
TAGACTC1201.508135E-610.2998695
TTAGGCA856.547418E-410.0665514
TCAGGAC3500.010.0502843
GTAGGAC13750.010.025593
ACCGTGC1151.0288937E-59.91099558
TGGCGAG12550.09.91059518
TCCTACA13050.09.9091252
GGCGAGG5550.09.7511119
CTATACT2550.09.6937164
GGTATCA14100.09.6532271
CTGTAGG14700.09.5182161
Produced by FastQC (version 0.11.5)