FastQCFastQC Report
Thu 26 May 2016
SRR1512743_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512743_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767841
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37230.21059586241070324No Hit
GTCCTACAGTGGACATTTCTAAATT27930.15798932143784425No Hit
GTCCTAAAGTGTGTATTTCTCATTT24300.1374558006065025No Hit
CTGTAGGACGTGGAATATGGCAAGA23950.13547598454838417No Hit
GGTATCAACGCAGAGTACTTTTTTT22130.12518094104616875No Hit
CTTTAGGACGTGAAATATGGCGAGG20730.11726167681369536No Hit
TATCAACGCAGAGTACTTTTTTTTT18630.10538278046498527No Hit
GTCCTACAGTGTGCATTTCTCATTT18540.10487368490718339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT453.5484656E-516.88384
TAGGACC5800.012.9357564
GGGCGTC550.003093405912.07797911
GTGTAGG3700.011.9615581
GTCTTAT1104.2172178E-711.3705721
GTCCTAG2600.011.1014461
GTAGGAC18350.011.0245543
GTCCTAC15350.010.8434971
GTATAGG1802.7284841E-1110.6902821
TGTAGGA20200.010.6731342
GGCGAGG6500.010.6629819
TCCTACA17000.010.4474482
TGGCGAG14550.010.43942618
TAGGACA5100.010.428234
TTATACT2100.010.4016284
ATTATAC1752.3646862E-1010.3117663
TCCAACG1201.5373917E-610.28446718
AATCCCG1856.002665E-1110.26425419
AAATCCC2500.010.25282218
GCGCAGA951.4464847E-410.1276351
Produced by FastQC (version 0.11.5)