FastQCFastQC Report
Thu 26 May 2016
SRR1512743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767841
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41920.2371253975894891No Hit
GTCCTACAGTGGACATTTCTAAATT29890.16907629136330699No Hit
CTGTAGGACGTGGAATATGGCAAGA27290.15436908636014213No Hit
GGTATCAACGCAGAGTACTTTTTTT26970.15255896882129105No Hit
GTCCTAAAGTGTGTATTTCTCATTT25220.1426598885306993No Hit
CTTTAGGACGTGAAATATGGCGAGG24270.1372861020872352No Hit
TATCAACGCAGAGTACTTTTTTTTT24120.13643760949089878No Hit
GTCCTACAGTGTGCATTTCTCATTT19290.10911614788886558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA350.002173101516.28263310
CCAACGT802.0136686E-613.05448719
TAGGACC5550.013.0187064
CGTGCGC604.0990533E-412.6642710
TCCAACG1701.8189894E-1211.72809518
GTATTAG1406.566552E-1011.5600311
GTATTAT2150.011.51258851
TGCACCG752.0592732E-411.4088215
CTACACG752.0598152E-411.4084984
AGGACCT10200.011.2783285
GCGCCAC855.3430835E-511.1724613
TAGGACT2650.011.1214914
TAGGACA5500.011.0627854
ACTGCGC951.3632309E-510.9979198
GTAGGAC17500.010.9192523
TATTATA2550.010.8132012
GGACCTG10250.010.7494916
TTAGGAC8850.010.5273173
CTGTAGG18450.010.5262061
TGTAGGA18900.010.4136982
Produced by FastQC (version 0.11.5)