FastQCFastQC Report
Thu 26 May 2016
SRR1512742_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512742_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118811
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30380.2715382669637678No Hit
GGTATCAACGCAGAGTACTTTTTTT19490.1742027920712256No Hit
TATCAACGCAGAGTACTTTTTTTTT16790.15007002970117383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC456.785483E-414.7704983
TTACTAC602.5789139E-514.2429813
GCACCGT500.001505164413.29285056
GGTATCA6700.012.9170621
GCCTAAC550.002800768512.2385891
GTATTAG958.971092E-712.146571
TTATACT1302.6338967E-911.6854964
GGTATAG751.8516555E-411.5392421
TAGGACC855.3511278E-511.169964
GTGTAGG1900.011.1343561
CTGATAC951.3665591E-510.9945823
CTAGACA1303.2889147E-810.95515254
CCTACAC700.001496963210.8517943
CTAGGAC1408.400093E-910.8517943
GTCCACG803.3686744E-410.8180391
TGGTACT1158.8723755E-710.7328754
TAGGGGT803.789199E-410.6812744
TCTAGGA1951.4551915E-1110.2257292
TTAAACC750.002659379710.127434
CCTAGAC1054.1236097E-59.9474783
Produced by FastQC (version 0.11.5)