FastQCFastQC Report
Thu 26 May 2016
SRR1512742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118811
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37690.33687548656564875No Hit
GGTATCAACGCAGAGTACTTTTTTT24700.2207700853852885No Hit
TATCAACGCAGAGTACTTTTTTTTT23070.20620104736188685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG400.005283815314.24758411
GGTATCA7200.012.4262411
TAGGAGT1354.652975E-911.2694474
ATAGGAC1104.905105E-711.2374333
GTCCTAG1201.2407145E-711.10430051
TAAGCTG1158.694187E-710.749815
AAAGGCG803.7344245E-410.6981295
GTGCGCC803.774798E-410.68568811
TAGGACG3500.010.5952934
GTCCTAC3350.010.5123871
TGTAATA1451.4204488E-810.4922452
CTTATAC1002.3472383E-510.4697691
GTATAGC1201.4853194E-610.3111361
ATAGACT1201.5042242E-610.300984
GTGTAGG1951.4551915E-1110.2501231
GTATCAA14500.010.2400251
AGGACGT3550.010.1791275
TTCACGG750.002626790410.1425032
GTCTAAT856.476328E-410.0778521
GGGTTAC856.476328E-410.0778521
Produced by FastQC (version 0.11.5)