FastQCFastQC Report
Thu 26 May 2016
SRR1512741_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512741_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665862
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46840.281175751652898No Hit
GGTATCAACGCAGAGTACTTTTTTT28710.17234320730048466No Hit
TATCAACGCAGAGTACTTTTTTTTT25450.15277375917092773No Hit
GTCCTACAGTGGACATTTCTAAATT22590.135605470321071No Hit
GTCCTAAAGTGTGTATTTCTCATTT21960.13182364445554312No Hit
CTGTAGGACGTGGAATATGGCAAGA19600.11765680470531173No Hit
CTTTAGGACGTGAAATATGGCGAGG19530.11723660183136418No Hit
GTACATGGGAAGCAGTGGTATCAAC17680.10613124016275058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGA1302.7130227E-811.1003791
GTATTAA2400.010.8228681
TTAAACC1951.8189894E-1210.7140994
GGCGAGG6750.010.6895519
GGACGTG13750.010.5664746
AGGACGT14050.010.5436185
TAGGACC3750.010.38293654
GTCTAGG1303.2308162E-710.3603531
TGTAGGA13900.010.3170612
GTATTAC1803.1286618E-1010.1547911
GTAGGAC13350.010.030713
ACCGTGC856.831294E-410.0201168
TTAGGAC8950.09.9746723
GTATTAG1553.342393E-89.9306611
GACGTGG7000.09.9029877
CGTGGAA6800.09.8958359
TAGGACG15450.09.7731884
GGTATCA13100.09.7672031
GTCCTAC11400.09.7047171
GTCCTAG1908.367351E-109.6203281
Produced by FastQC (version 0.11.5)