FastQCFastQC Report
Thu 26 May 2016
SRR1512741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665862
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58060.34852826944848975No Hit
TATCAACGCAGAGTACTTTTTTTTT35230.21148210355959857No Hit
GGTATCAACGCAGAGTACTTTTTTT35220.21142207457760606No Hit
GTCCTACAGTGGACATTTCTAAATT22780.13674602097892863No Hit
GTCCTAAAGTGTGTATTTCTCATTT21880.13134341259960308No Hit
CTGTAGGACGTGGAATATGGCAAGA21710.13032291990573047No Hit
CTTTAGGACGTGAAATATGGCGAGG21110.12672118098618013No Hit
GTACATGGGAAGCAGTGGTATCAAC20460.12281929715666723No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16710.1003084289094775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC400.00528418614.2480186
ACTGCGC500.001501198913.298158
TAGGACC4200.011.5441644
CAGGTCG600.00588204411.0817919
GGCGAGG5450.010.97452119
ACCGTGC803.7758282E-410.6860138
GATTTAC909.3330375E-510.57568551
GGACGTG13000.010.3024136
GTCCTAA7500.010.0257491
AGGACGT13400.010.0041925
TGTAGGA13400.09.9338372
TCGAACT1356.498449E-79.84545519
GTAGGAC12900.09.8017483
TGGCGAG11450.09.78403618
TTATACT1856.4028427E-109.7639044
GTGTTAC2054.0017767E-119.7502651
GTATAGA2151.0913936E-119.7394691
GGTATCA14600.09.7136951
GACGTGG6450.09.5723117
GTATTAG1804.0436134E-99.5181171
Produced by FastQC (version 0.11.5)