FastQCFastQC Report
Thu 26 May 2016
SRR1512740_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512740_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57223
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC29755.198958460758786No Hit
CCCATGTACTCTGCGTTGATACCAC18933.3081103751987833No Hit
GAGTACATGGGAAGCAGTGGTATCA14162.474529472414938No Hit
GTATCAACGCAGAGTACTTTTTTTT13972.4413260402285792No Hit
GGTATCAACGCAGAGTACTTTTTTT10981.9188088705590407No Hit
TATCAACGCAGAGTACTTTTTTTTT9211.6094926865071735No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8421.4714363105744195No Hit
CATGTACTCTGCGTTGATACCACTG7701.3456127780787446No Hit
GTATCAACGCAGAGTACATGGGAAG7511.3124093458923858No Hit
ACGCAGAGTACATGGGAAGCAGTGG7461.3036716005801863No Hit
TATCAACGCAGAGTACATGGGAAGC7311.2774583646435875No Hit
GGTATCAACGCAGAGTACATGGGAA7241.2652255212065078No Hit
GCGTTGATACCACTGCTTCCCATGT6781.1848382643342712No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5781.0100833580902784No Hit
ACTCTGCGTTGATACCACTGCTTCC5340.9331911993429216No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5040.8807647274697237No Hit
GTGGTATCAACGCAGAGTACATGGG4610.8056201177848068No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4210.7357181552872097No Hit
GCTTCCCATGTACTCTGCGTTGATA4070.7112524684130507No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT3740.653583349352533No Hit
GGGAAGCAGTGGTATCAACGCAGAG3460.6046519756042151No Hit
GCAGTGGTATCAACGCAGAGTACAT3350.5854289359173759No Hit
GTACTCTGCGTTGATACCACTGCTT3170.5539730527934572No Hit
CATGGGAAGCAGTGGTATCAACGCA3090.5399926602939378No Hit
GCAGAGTACATGGGAAGCAGTGGTA2960.5172745224822187No Hit
ACATGGGAAGCAGTGGTATCAACGC2890.5050416790451392No Hit
CAGTGGTATCAACGCAGAGTACATG2750.4805759921709802No Hit
CTGCTTCCCATGTACTCTGCGTTGA2680.4683431487339007No Hit
AAGCAGTGGTATCAACGCAGAGTAC2520.44038236373486184No Hit
GATACCACTGCTTCCCATGTACTCT2470.4316446184226623No Hit
ATGGGAAGCAGTGGTATCAACGCAG2310.40368383342362335No Hit
ATACCACTGCTTCCCATGTACTCTG2300.40193628436118345No Hit
GTTGATACCACTGCTTCCCATGTAC2130.3722279502997047No Hit
ACCATGTACTCTGCGTTGATACCAC2050.35824755780018525No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2030.3547524596753054No Hit
CTTCCCATGTACTCTGCGTTGATAC1950.34077206717578595No Hit
TACCACTGCTTCCCATGTACTCTGC1820.3180539293640669No Hit
GTGGTATCAACGCAGAGTACTTTTT1810.31630638030162694No Hit
CCATGTACTCTGCGTTGATACCACT1740.30407353686454747No Hit
CCACTGCTTCCCATGTACTCTGCGT1720.3005784387396676No Hit
ATCAACGCAGAGTACATGGGAAGCA1580.27611275186550865No Hit
GTACATGGGGAAAAAAAAAAAAAAA1460.2551421631162295No Hit
GGTATCAACGCAGAGTACATGGGGA1400.2446568687415899No Hit
TGATACCACTGCTTCCCATGTACTC1380.24116177061671004No Hit
GCCATGTACTCTGCGTTGATACCAC1380.24116177061671004No Hit
GTACATGGTAAGCAGTGGTATCAAC1370.23941422155427014No Hit
TTCCCATGTACTCTGCGTTGATACC1330.23242402530451045No Hit
ATCAACGCAGAGTACTTTTTTTTTT1260.22019118186743092No Hit
GGAAGCAGTGGTATCAACGCAGAGT1240.2166960837425511No Hit
GGTATCAACGCAGAGTACATGGGGG1130.19747304405571187No Hit
GTATCAACGCAGAGTACATGGGGAA1000.17475490624399279No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA970.16951225905667303No Hit
ATACATGGGAAGCAGTGGTATCAAC950.16601716093179317No Hit
TCAACGCAGAGTACATGGGAAGCAG930.1625220628069133No Hit
GTACATGGGGAGGAAAAAAAAAAAA860.1502892193698338No Hit
GAGTACATGGGGAAAAAAAAAAAAA810.14155147405763416No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT780.1363088268703144No Hit
TGGGAAGCAGTGGTATCAACGCAGA750.1310661796829946No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT700.12232843437079495No Hit
GGTATCAACGCAGAGAAAAAAAAAA680.1188333362459151No Hit
GTACATGGGGGGTCTTGAGGGGTTT670.11708578718347518No Hit
ACGCAGAGTACATGGGGAAAAAAAA650.11359068905859532No Hit
GGTAAGCAGTGGTATCAACGCAGAG640.11184313999615539No Hit
GTACATGGGAAAAAAAAAAAAAAAA630.11009559093371546No Hit
CTACATGGGAAGCAGTGGTATCAAC630.11009559093371546No Hit
GTATCAACGCAGAGTACATGGGAAA620.10834804187127554No Hit
CTCTGCGTTGATACCACTGCTTCCC620.10834804187127554No Hit
ATATCAACGCAGAGTACTTTTTTTT620.10834804187127554No Hit
GTACATGGGTAAGCAGTGGTATCAA620.10834804187127554No Hit
AATGTACTCTGCGTTGATACCACTG610.10660049280883561No Hit
TATCAACGCAGAGTACATGGGGAAA610.10660049280883561No Hit
CAACGCAGAGTACTTTTTTTTTTTT600.10485294374639569No Hit
TCCCATGTACTCTGCGTTGATACCA590.10310539468395576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG359.0191075E-519.187391
CATGGGT1105.774589E-610.3554884
GAAGCAG8700.07.9649989
GGAAGCA8700.07.95102368
CCATGTA6000.07.9104422
CAGTGGT8800.07.881411613
AGCAGTG9400.07.876780511
AAGCAGT9300.07.859407410
AGTGGTA8850.07.836884514
TGATACC6300.07.540380517
ATGGGGA1408.9306943E-47.45841745
GGGAAGC9300.07.45112667
GCAGTGG9750.07.39930612
TTGATAC6450.07.36502316
GTGGTAT9550.07.262452615
TGGTATC9450.07.23876516
GATACCA6600.07.19763618
ATACCAC6650.07.14351819
CATGTAC6700.07.0839783
TGCGTTG6750.07.03150412
Produced by FastQC (version 0.11.5)