##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512740_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57223 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.919892350977754 31.0 25.0 33.0 10.0 34.0 2 28.249969417891407 31.0 27.0 34.0 16.0 34.0 3 29.382049176030616 31.0 28.0 34.0 22.0 34.0 4 33.27625255579051 35.0 35.0 37.0 28.0 37.0 5 33.67315589885186 37.0 35.0 37.0 30.0 37.0 6 34.105359732974506 37.0 35.0 37.0 31.0 37.0 7 34.08327071282526 37.0 35.0 37.0 31.0 37.0 8 34.175279170962725 37.0 35.0 37.0 32.0 37.0 9 35.68893626688569 39.0 37.0 39.0 31.0 39.0 10 35.676790800901735 39.0 37.0 39.0 31.0 39.0 11 35.72988134141866 39.0 37.0 39.0 31.0 39.0 12 35.61653530922881 39.0 37.0 39.0 30.0 39.0 13 35.54680810163745 39.0 37.0 39.0 30.0 39.0 14 36.64206700103105 40.0 38.0 41.0 30.0 41.0 15 36.40564108837356 40.0 37.0 41.0 30.0 41.0 16 36.118291596036556 40.0 37.0 41.0 28.0 41.0 17 36.42271464271359 40.0 37.0 41.0 30.0 41.0 18 36.3447215280569 40.0 37.0 41.0 30.0 41.0 19 36.260245006378554 40.0 37.0 41.0 30.0 41.0 20 36.40777309822973 40.0 37.0 41.0 30.0 41.0 21 36.287943659018225 40.0 37.0 41.0 30.0 41.0 22 36.259213952431715 40.0 37.0 41.0 30.0 41.0 23 36.080666864722225 40.0 36.0 41.0 29.0 41.0 24 36.02588120161474 40.0 36.0 41.0 29.0 41.0 25 35.919892350977754 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.9363168788387206 1101 2 2.8470320010801125 1101 3 1.3665425593008393 1101 4 1.6467459278822218 1101 5 1.5302760865838891 1101 6 -0.04045048822340647 1101 7 -0.29311763458670015 1101 8 0.1564647403174746 1101 9 0.1702238012625159 1101 10 -0.7065801117058328 1101 11 -0.4761857880048197 1101 12 -1.0202471017841717 1101 13 -0.3870466245959392 1101 14 -1.1697205797073522 1101 15 -0.837336615640119 1101 16 -1.3303998351147541 1101 17 -0.6240146894814202 1101 18 -0.964917500755071 1101 19 -0.6875935824248245 1101 20 -0.5868756598841074 1101 21 -1.3068738593662417 1101 22 -1.6747733776981377 1101 23 -1.254366587413621 1101 24 -1.5311482671666923 1101 25 -2.4734208680759266 1102 1 1.5404835455053885 1102 2 0.9928653344134446 1102 3 -0.6334574406991607 1102 4 -0.6324207387844396 1102 5 -0.8738905800827723 1102 6 -1.4029504882234036 1102 7 -1.2139509679200344 1102 8 -1.6102019263491911 1102 9 -1.333942865404147 1102 10 -1.6607467783724985 1102 11 -1.2178524546714868 1102 12 -2.1494137684508345 1102 13 -1.466213291262605 1102 14 -2.2905539130406893 1102 15 -1.1373366156401161 1102 16 -2.28873316844809 1102 17 -2.4031813561480817 1102 18 -1.8690841674217324 1102 19 -2.412593582424826 1102 20 -1.6243756598841088 1102 21 -1.4443738593662445 1102 22 -1.8039400443648006 1102 23 -1.5751999207469538 1102 24 -2.110314933833358 1102 25 -3.0567542014092624 1103 1 1.378624571146414 1103 2 -0.0760449219968109 1103 3 -1.4603805176222373 1103 4 -3.0474848413485454 1103 5 -2.5678008364930314 1103 6 -1.9366043343772503 1103 7 -2.491194557663622 1103 8 -2.2896891058363735 1103 9 -2.3643915833528695 1103 10 -1.816195496321221 1103 11 -2.4165704033894357 1103 12 -3.516400947938017 1103 13 -3.0351235476728604 1103 14 -3.962028272015047 1103 15 -3.7431058464093496 1103 16 -3.7246306043455206 1103 17 -3.5874762279429575 1103 18 -2.8033790392166082 1103 19 -3.5856705055017457 1103 20 -3.0714910444994956 1103 21 -3.4587969362893247 1103 22 -2.6901579930827495 1103 23 -4.467828125875158 1103 24 -4.688840574859 1103 25 -4.242651637306697 1104 1 -8.43632463059524 1104 2 -10.360515168731208 1104 3 -9.66647630862369 1104 4 -8.599008789098907 1104 5 -6.229629573793467 1104 6 -4.392337280676234 1104 7 -3.502079898737641 1104 8 -2.8539126181730943 1104 9 -3.4467573308129573 1104 10 -2.56837256453602 1104 11 -3.838921637061425 1104 12 -2.5381716300860546 1104 13 -3.3667636057280177 1104 14 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2.024662801654351 2314 21 1.2142799867876022 2314 22 1.6559958530710972 2314 23 1.4937103356633017 2314 24 1.0226978866794596 2314 25 0.8535022088471536 2315 1 0.5238168788387192 2315 2 1.7720320010801132 2315 3 1.29154255930084 2315 4 1.0842459278822218 2315 5 1.3177760865838906 2315 6 1.2595495117765978 2315 7 1.2193823654133027 2315 8 0.8064647403174732 2315 9 0.19522380126251448 2315 10 0.5184198882941686 2315 11 0.5488142119951789 2315 12 0.5797528982158298 2315 13 0.3254533754040594 2315 14 1.067779420292645 2315 15 0.4376633843598867 2315 16 0.5821001648852473 2315 17 0.46348531051858544 2315 18 0.4975824992449347 2315 19 0.32490641757517125 2315 20 0.11312434011588834 2315 21 0.41812614063375264 2315 22 -1.1247733776981335 2315 23 0.20813341258638474 2315 24 -0.6311482671666937 2315 25 -0.12342086807592523 2316 1 1.419650212172055 2316 2 1.2914764455245553 2316 3 1.238764781523063 2316 4 1.0439681501044475 2316 5 0.855276086583892 2316 6 0.37621617844326494 2316 7 0.6207712543021913 2316 8 0.3620202958730303 2316 9 0.3146682457069616 2316 10 0.3406421105163915 2316 11 0.021036434217400313 2316 12 0.3186417871047169 2316 13 0.11017559762628082 2316 14 0.31916830918153494 2316 15 0.38766338435988246 2316 16 0.016822387107467307 2316 17 0.39542975496302546 2316 18 0.4517491659116004 2316 19 0.43185086201961553 2316 20 0.3075687845603383 2316 21 -0.2818738593662431 2316 22 0.1974488445240894 2316 23 -0.19047769852473095 2316 24 0.19107395505552915 2316 25 -0.04008753474259663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2281.0 3 0.0 4 9.0 5 63.0 6 67.0 7 59.0 8 87.0 9 61.0 10 53.0 11 63.0 12 68.0 13 72.0 14 73.0 15 50.0 16 68.0 17 61.0 18 94.0 19 107.0 20 104.0 21 143.0 22 146.0 23 183.0 24 197.0 25 274.0 26 274.0 27 329.0 28 395.0 29 489.0 30 678.0 31 838.0 32 1168.0 33 1763.0 34 2725.0 35 4222.0 36 7906.0 37 10899.0 38 21154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.80309868163889 19.880245904552076 11.124338154294446 18.19231725951459 2 13.85232829176817 24.576315743353373 36.686587567254946 24.884768397623514 3 23.118298409138692 33.083958231130424 27.33197841474525 16.465764944985633 4 13.024812504380739 20.5211326838158 30.42685918553305 36.02719562627041 5 10.030315243485727 43.00208526819353 31.988715019187975 14.978884469132774 6 28.737020889145697 30.782161054788045 20.45315263259267 20.027665423473586 7 23.22071083808688 37.06888986397543 20.9811320754717 18.729267222465996 8 31.65481486671104 28.615966651487025 21.29996146705433 18.42925701474761 9 32.522019296433136 13.992540580293825 19.354217373793972 34.131222749479065 10 21.780618051300006 31.686947386851088 26.754792961568764 19.777641600280134 11 27.583670467083486 28.37878071419815 17.64128968983695 26.396259128881418 12 28.372532880334845 33.58960438521217 25.027582704330925 13.010280030122065 13 31.619694873097338 22.674326951708675 22.896778826785308 22.809199348408683 14 24.720124739396276 29.72371625291263 26.1760016818795 19.380157325811595 15 34.67836770513383 22.037735849056602 25.146116717858707 18.137779727950857 16 16.226090316688037 29.453919519435125 34.645987388684944 19.674002775191894 17 24.800646362454774 30.349176239154108 26.792426318192998 18.057751080198123 18 23.350502820701497 23.541469567959634 33.83089806930867 19.277129542030206 19 21.10784210710658 29.87285909425239 36.30520822388007 12.714090574760952 20 23.966594884185096 25.367228670973617 32.40541344958594 18.26076299525535 21 19.892718157276583 26.948427584756164 36.95263471584335 16.2062195421239 22 22.000525992811433 27.66196195318664 27.263960725870078 23.07355132813185 23 16.89920848031731 29.66531529159866 33.493041295916036 19.94243493216799 24 19.36292556361979 35.24946530626556 29.094351530451245 16.293257599663406 25 19.547224073229756 26.35815242170238 28.159085329498822 25.93553817556904 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1371.0 1 1371.0 2 932.0 3 493.0 4 493.0 5 493.0 6 637.0 7 781.0 8 781.0 9 781.0 10 679.5 11 578.0 12 578.0 13 578.0 14 419.5 15 261.0 16 261.0 17 261.0 18 486.5 19 712.0 20 712.0 21 712.0 22 1138.5 23 1565.0 24 1565.0 25 1565.0 26 2084.5 27 2604.0 28 2604.0 29 2604.0 30 2905.5 31 3207.0 32 3207.0 33 3207.0 34 3095.5 35 2984.0 36 2984.0 37 2984.0 38 2767.5 39 2551.0 40 2551.0 41 2551.0 42 3340.5 43 4130.0 44 4130.0 45 4130.0 46 9745.5 47 15361.0 48 15361.0 49 15361.0 50 12849.5 51 10338.0 52 10338.0 53 10338.0 54 7490.0 55 4642.0 56 4642.0 57 4642.0 58 3447.0 59 2252.0 60 2252.0 61 2252.0 62 1886.0 63 1520.0 64 1520.0 65 1520.0 66 1229.0 67 938.0 68 938.0 69 938.0 70 707.5 71 477.0 72 477.0 73 477.0 74 357.5 75 238.0 76 238.0 77 238.0 78 183.0 79 128.0 80 128.0 81 128.0 82 90.5 83 53.0 84 53.0 85 53.0 86 34.0 87 15.0 88 15.0 89 15.0 90 14.0 91 13.0 92 13.0 93 13.0 94 9.5 95 6.0 96 6.0 97 6.0 98 5.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.6444436677559724 2 0.2865980462401482 3 0.2568897121786694 4 0.27087010467818884 5 0.2726176537406288 6 0.19572549499327196 7 0.4333921674851022 8 0.22543382905475073 9 0.19922059311815182 10 0.18873529874351222 11 0.2166960837425511 12 0.21320098561767123 13 0.2306764762420705 14 0.25164706499134964 15 0.4333921674851022 16 0.5067892281075791 17 0.5050416790451392 18 0.25339461405378955 19 0.21145343655523127 20 0.1852402006186324 21 0.3093161840518672 22 0.3285392237387065 23 0.42640197123534246 24 0.31630638030162694 25 0.3442671653006658 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 57223.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.82919455463712 #Duplication Level Percentage of deduplicated Percentage of total 1 86.02115766618931 32.5411110916939 2 8.024206587517902 6.07098544291631 3 2.2866909964429247 2.5951103577232932 4 0.9146763985771701 1.3840588574524229 5 0.5451101769298287 1.0310539468395574 6 0.30489213285905664 0.6920294287262114 7 0.22173973298840488 0.5871764849798158 8 0.13396775534716127 0.4054313824860633 9 0.1293481775765695 0.44038236373486184 >10 1.0301658428419642 7.50921832130437 >50 0.15706564420012012 3.938975586739598 >100 0.15706564420012012 13.86854935952327 >500 0.05081535547650944 13.594184156720198 >1k 0.02309788885295884 15.341733219160128 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2975 5.198958460758786 No Hit CCCATGTACTCTGCGTTGATACCAC 1893 3.3081103751987833 No Hit GAGTACATGGGAAGCAGTGGTATCA 1416 2.474529472414938 No Hit GTATCAACGCAGAGTACTTTTTTTT 1397 2.4413260402285792 No Hit GGTATCAACGCAGAGTACTTTTTTT 1098 1.9188088705590407 No Hit TATCAACGCAGAGTACTTTTTTTTT 921 1.6094926865071735 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 842 1.4714363105744195 No Hit CATGTACTCTGCGTTGATACCACTG 770 1.3456127780787446 No Hit GTATCAACGCAGAGTACATGGGAAG 751 1.3124093458923858 No Hit ACGCAGAGTACATGGGAAGCAGTGG 746 1.3036716005801863 No Hit TATCAACGCAGAGTACATGGGAAGC 731 1.2774583646435875 No Hit GGTATCAACGCAGAGTACATGGGAA 724 1.2652255212065078 No Hit GCGTTGATACCACTGCTTCCCATGT 678 1.1848382643342712 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 578 1.0100833580902784 No Hit ACTCTGCGTTGATACCACTGCTTCC 534 0.9331911993429216 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 504 0.8807647274697237 No Hit GTGGTATCAACGCAGAGTACATGGG 461 0.8056201177848068 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 421 0.7357181552872097 No Hit GCTTCCCATGTACTCTGCGTTGATA 407 0.7112524684130507 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 374 0.653583349352533 No Hit GGGAAGCAGTGGTATCAACGCAGAG 346 0.6046519756042151 No Hit GCAGTGGTATCAACGCAGAGTACAT 335 0.5854289359173759 No Hit GTACTCTGCGTTGATACCACTGCTT 317 0.5539730527934572 No Hit CATGGGAAGCAGTGGTATCAACGCA 309 0.5399926602939378 No Hit GCAGAGTACATGGGAAGCAGTGGTA 296 0.5172745224822187 No Hit ACATGGGAAGCAGTGGTATCAACGC 289 0.5050416790451392 No Hit CAGTGGTATCAACGCAGAGTACATG 275 0.4805759921709802 No Hit CTGCTTCCCATGTACTCTGCGTTGA 268 0.4683431487339007 No Hit AAGCAGTGGTATCAACGCAGAGTAC 252 0.44038236373486184 No Hit GATACCACTGCTTCCCATGTACTCT 247 0.4316446184226623 No Hit ATGGGAAGCAGTGGTATCAACGCAG 231 0.40368383342362335 No Hit ATACCACTGCTTCCCATGTACTCTG 230 0.40193628436118345 No Hit GTTGATACCACTGCTTCCCATGTAC 213 0.3722279502997047 No Hit ACCATGTACTCTGCGTTGATACCAC 205 0.35824755780018525 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 203 0.3547524596753054 No Hit CTTCCCATGTACTCTGCGTTGATAC 195 0.34077206717578595 No Hit TACCACTGCTTCCCATGTACTCTGC 182 0.3180539293640669 No Hit GTGGTATCAACGCAGAGTACTTTTT 181 0.31630638030162694 No Hit CCATGTACTCTGCGTTGATACCACT 174 0.30407353686454747 No Hit CCACTGCTTCCCATGTACTCTGCGT 172 0.3005784387396676 No Hit ATCAACGCAGAGTACATGGGAAGCA 158 0.27611275186550865 No Hit GTACATGGGGAAAAAAAAAAAAAAA 146 0.2551421631162295 No Hit GGTATCAACGCAGAGTACATGGGGA 140 0.2446568687415899 No Hit TGATACCACTGCTTCCCATGTACTC 138 0.24116177061671004 No Hit GCCATGTACTCTGCGTTGATACCAC 138 0.24116177061671004 No Hit GTACATGGTAAGCAGTGGTATCAAC 137 0.23941422155427014 No Hit TTCCCATGTACTCTGCGTTGATACC 133 0.23242402530451045 No Hit ATCAACGCAGAGTACTTTTTTTTTT 126 0.22019118186743092 No Hit GGAAGCAGTGGTATCAACGCAGAGT 124 0.2166960837425511 No Hit GGTATCAACGCAGAGTACATGGGGG 113 0.19747304405571187 No Hit GTATCAACGCAGAGTACATGGGGAA 100 0.17475490624399279 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 97 0.16951225905667303 No Hit ATACATGGGAAGCAGTGGTATCAAC 95 0.16601716093179317 No Hit TCAACGCAGAGTACATGGGAAGCAG 93 0.1625220628069133 No Hit GTACATGGGGAGGAAAAAAAAAAAA 86 0.1502892193698338 No Hit GAGTACATGGGGAAAAAAAAAAAAA 81 0.14155147405763416 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 78 0.1363088268703144 No Hit TGGGAAGCAGTGGTATCAACGCAGA 75 0.1310661796829946 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 70 0.12232843437079495 No Hit GGTATCAACGCAGAGAAAAAAAAAA 68 0.1188333362459151 No Hit GTACATGGGGGGTCTTGAGGGGTTT 67 0.11708578718347518 No Hit ACGCAGAGTACATGGGGAAAAAAAA 65 0.11359068905859532 No Hit GGTAAGCAGTGGTATCAACGCAGAG 64 0.11184313999615539 No Hit GTACATGGGAAAAAAAAAAAAAAAA 63 0.11009559093371546 No Hit CTACATGGGAAGCAGTGGTATCAAC 63 0.11009559093371546 No Hit GTATCAACGCAGAGTACATGGGAAA 62 0.10834804187127554 No Hit CTCTGCGTTGATACCACTGCTTCCC 62 0.10834804187127554 No Hit ATATCAACGCAGAGTACTTTTTTTT 62 0.10834804187127554 No Hit GTACATGGGTAAGCAGTGGTATCAA 62 0.10834804187127554 No Hit AATGTACTCTGCGTTGATACCACTG 61 0.10660049280883561 No Hit TATCAACGCAGAGTACATGGGGAAA 61 0.10660049280883561 No Hit CAACGCAGAGTACTTTTTTTTTTTT 60 0.10485294374639569 No Hit TCCCATGTACTCTGCGTTGATACCA 59 0.10310539468395576 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACATG 35 9.0191075E-5 19.18739 1 CATGGGT 110 5.774589E-6 10.355488 4 GAAGCAG 870 0.0 7.964998 9 GGAAGCA 870 0.0 7.9510236 8 CCATGTA 600 0.0 7.910442 2 CAGTGGT 880 0.0 7.8814116 13 AGCAGTG 940 0.0 7.8767805 11 AAGCAGT 930 0.0 7.8594074 10 AGTGGTA 885 0.0 7.8368845 14 TGATACC 630 0.0 7.5403805 17 ATGGGGA 140 8.9306943E-4 7.4584174 5 GGGAAGC 930 0.0 7.4511266 7 GCAGTGG 975 0.0 7.399306 12 TTGATAC 645 0.0 7.365023 16 GTGGTAT 955 0.0 7.2624526 15 TGGTATC 945 0.0 7.238765 16 GATACCA 660 0.0 7.197636 18 ATACCAC 665 0.0 7.143518 19 CATGTAC 670 0.0 7.083978 3 TGCGTTG 675 0.0 7.031504 12 >>END_MODULE