FastQCFastQC Report
Thu 26 May 2016
SRR1512740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57223
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC33245.8088530835503205No Hit
CCCATGTACTCTGCGTTGATACCAC20503.5824755780018522No Hit
GTATCAACGCAGAGTACTTTTTTTT16842.942872621148839No Hit
GAGTACATGGGAAGCAGTGGTATCA15922.7820981074043654No Hit
GGTATCAACGCAGAGTACTTTTTTT13262.317250056795345No Hit
TATCAACGCAGAGTACTTTTTTTTT12082.111039267427433No Hit
TATCAACGCAGAGTACATGGGAAGC9181.6042500393198538No Hit
CATGTACTCTGCGTTGATACCACTG8991.5710466071334952No Hit
ACGCAGAGTACATGGGAAGCAGTGG8781.5343480768222568No Hit
GGTATCAACGCAGAGTACATGGGAA8701.5203676843227374No Hit
GTATCAACGCAGAGTACATGGGAAG8551.4941544483861384No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7591.3263897383919052No Hit
GCGTTGATACCACTGCTTCCCATGT7121.2442549324572287No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6591.1516348321479126No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6511.1376544396483932No Hit
ACTCTGCGTTGATACCACTGCTTCC5440.9506666899673208No Hit
GTGGTATCAACGCAGAGTACATGGG5240.9157157087185224No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4970.8685318840326443No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT4690.8196005102843262No Hit
GGGAAGCAGTGGTATCAACGCAGAG4320.7549411949740489No Hit
GCTTCCCATGTACTCTGCGTTGATA3980.6955245268510913No Hit
CATGGGAAGCAGTGGTATCAACGCA3960.6920294287262114No Hit
CAGTGGTATCAACGCAGAGTACATG3920.6850392324764518No Hit
GCAGTGGTATCAACGCAGAGTACAT3450.6029044265417752No Hit
ACATGGGAAGCAGTGGTATCAACGC3220.5627107981056568No Hit
GCAGAGTACATGGGAAGCAGTGGTA3090.5399926602939378No Hit
GTACTCTGCGTTGATACCACTGCTT3080.5382451112314979No Hit
ATACCACTGCTTCCCATGTACTCTG2890.5050416790451392No Hit
ATGGGAAGCAGTGGTATCAACGCAG2790.4875661884207399No Hit
GATACCACTGCTTCCCATGTACTCT2710.47358579592122046No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2660.4648480506090209No Hit
AAGCAGTGGTATCAACGCAGAGTAC2630.4596054034217011No Hit
CTGCTTCCCATGTACTCTGCGTTGA2470.4316446184226623No Hit
TACCACTGCTTCCCATGTACTCTGC2330.4071789315485032No Hit
GTTGATACCACTGCTTCCCATGTAC2310.40368383342362335No Hit
CTTCCCATGTACTCTGCGTTGATAC2100.3669853031123849No Hit
CCACTGCTTCCCATGTACTCTGCGT2050.35824755780018525No Hit
GTGGTATCAACGCAGAGTACTTTTT1990.34776226342554567No Hit
CTTATACACATCTCCGAGCCCACGA1950.34077206717578595No Hit
CCATGTACTCTGCGTTGATACCACT1940.33902451811334605No Hit
ATCAACGCAGAGTACATGGGAAGCA1720.3005784387396676No Hit
GGAAGCAGTGGTATCAACGCAGAGT1660.29009314436502803No Hit
ATCAACGCAGAGTACTTTTTTTTTT1550.27087010467818884No Hit
TGATACCACTGCTTCCCATGTACTC1520.2656274574908691No Hit
TTCCCATGTACTCTGCGTTGATACC1440.25164706499134964No Hit
GTACATGGTAAGCAGTGGTATCAAC1420.24815196686646976No Hit
GTACATGGGGAAAAAAAAAAAAAAA1330.23242402530451045No Hit
GGTATCAACGCAGAGTACATGGGGA1320.2306764762420705No Hit
ATACACATCTCCGAGCCCACGAGAC1310.2289289271796306No Hit
GGTATCAACGCAGAGTACATGGGGG1110.193977945930832No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1060.1852402006186324No Hit
GTATCAACGCAGAGTACATGGGGAA990.17300735718155286No Hit
ACACATCTCCGAGCCCACGAGACTA970.16951225905667303No Hit
ACCATGTACTCTGCGTTGATACCAC940.16426961186935324No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT920.16077451374447338No Hit
TCAACGCAGAGTACATGGGAAGCAG900.15727941561959352No Hit
TGGGAAGCAGTGGTATCAACGCAGA850.14854167030739387No Hit
GGTATCAACGCAGAGAAAAAAAAAA800.13980392499519423No Hit
AACGCAGAGTACTTTTTTTTTTTTT750.1310661796829946No Hit
TCAACGCAGAGTACTTTTTTTTTTT740.12931863062055468No Hit
GTACATGGGTAAGCAGTGGTATCAA720.12582353249567482No Hit
AACGCAGAGTACATGGGAAGCAGTG710.12407598343323488No Hit
AGTGGTATCAACGCAGAGTACATGG670.11708578718347518No Hit
GGTAAGCAGTGGTATCAACGCAGAG670.11708578718347518No Hit
TCCCATGTACTCTGCGTTGATACCA660.11533823812103525No Hit
GAGTACATGGGGAAAAAAAAAAAAA660.11533823812103525No Hit
CCCCATGTACTCTGCGTTGATACCA660.11533823812103525No Hit
ACGCAGAGTACATGGGGAAAAAAAA650.11359068905859532No Hit
AAAAAGTACTCTGCGTTGATACCAC650.11359068905859532No Hit
CAACGCAGAGTACTTTTTTTTTTTT630.11009559093371546No Hit
AAAGTACTCTGCGTTGATACCACTG620.10834804187127554No Hit
TATCAACGCAGAGTACATGGGGAAA610.10660049280883561No Hit
GTACATGGGGGGTCTTGAGGGGTTT600.10485294374639569No Hit
CTGCGTTGATACCACTGCTTCCCAT590.10310539468395576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC250.00585220119.0615121
CCATGTA5600.08.1548872
TTGATAC6100.07.77792116
TGATACC6300.07.68162317
GATACCA6350.07.621138618
AGTGGTA9350.07.510061714
ATACCAC6450.07.502980719
GAAGCAG8750.07.4893579
TGCGTTG6550.07.39490112
CAGTGGT9400.07.3756213
GGAAGCA8900.07.36313158
TCTGCGT6650.07.283699510
AGCAGTG9650.07.282959511
CCCATGT5650.07.2534951
GTTGATA6550.07.2435615
CGTTGAT6550.07.2435614
CTGCGTT6700.07.229343411
AAGCAGT9600.07.22196110
CTCTGCG6850.07.2096859
TACTCTG6750.07.1757937
Produced by FastQC (version 0.11.5)