FastQCFastQC Report
Thu 26 May 2016
SRR1512739_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512739_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904856
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42470.22295648594959408No Hit
GGTATCAACGCAGAGTACTTTTTTT25510.13392088430831517No Hit
TATCAACGCAGAGTACTTTTTTTTT21290.11176697871125166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCAA752.0874575E-411.39222410
GACGCTC752.1433737E-411.3598428
GTAGGAC7650.010.9212273
AGGACGT7250.010.86844355
GTGTAGG3900.010.8538781
AAGTCGT803.7962737E-410.6799297
GTCCTAC7550.010.576161
TGTAGGA9300.010.4127982
TAGGACG7700.010.3568414
GGCGAGG3950.010.33580919
ATTATTC1752.382876E-1010.3077953
GGACGTG7400.010.2629956
TAGACGA750.002663058710.1264225
TAAGACT2350.010.0997564
TAGGACC3150.09.9458554
GACGTGA3950.09.8537747
TTAGGAC5300.09.8522853
GGTATCA12300.09.7769141
TGGCGAG7600.09.742578518
GTCCTAA4950.09.717661
Produced by FastQC (version 0.11.5)