FastQCFastQC Report
Thu 26 May 2016
SRR1512739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904856
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50250.26379946830626566No Hit
GGTATCAACGCAGAGTACTTTTTTT32210.16909414674915058No Hit
TATCAACGCAGAGTACTTTTTTTTT28970.15208498700164214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATG600.00588113711.0821548
GAGCCGT700.00149364910.8554166
GGCGAGG3700.010.77754119
GTATTAA2050.010.6753461
TAGGACC4100.010.6655254
TTAGGAC5550.010.6195723
AGGACGT7900.010.5892185
GTCCTAG2250.010.5722021
GTAGGAC8100.010.5624763
AATCCCG1002.4145313E-510.44395119
GGACGTG7750.010.4176986
GTCCTAC6800.010.3545391
TAGGACG8100.010.3277554
CTAGACT1606.1063474E-910.10036854
CTGTAGG10100.09.7033981
GACGTGA4650.09.6011297
TGTAGGA9450.09.5567782
CTAGATC900.00110709829.5062294
TAGGACA3400.09.5062294
GACCGGC1002.7585155E-49.4989887
Produced by FastQC (version 0.11.5)