FastQCFastQC Report
Thu 26 May 2016
SRR1512738_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512738_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728966
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40970.23696243882181606No Hit
GTCCTACAGTGGACATTTCTAAATT25990.15032105894505732No Hit
GGTATCAACGCAGAGTACTTTTTTT23780.13753885270155689No Hit
CTGTAGGACGTGGAATATGGCAAGA22550.13042477411354533No Hit
TATCAACGCAGAGTACTTTTTTTTT22340.12921017533022627No Hit
GTCCTAAAGTGTGTATTTCTCATTT22050.12753287224849996No Hit
CTTTAGGACGTGAAATATGGCGAGG18430.10659550274557163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG400.004856361514.4304721
TAGGACC5450.013.0662164
AGGCCCG802.0121242E-613.0553275
CCAACGA1102.7721399E-912.9466819
GCACCGT604.1124359E-412.6593436
AGGACCT11400.011.7435485
GTGTAGG3200.011.7247591
GGACCTG10900.011.1495146
TGTAGGA16600.010.8109532
TGGCGAG14050.010.74311318
GGCGAGG5300.010.56905119
ATGTAGG2650.010.5278911
GGTATCA10250.010.5119531
GTCCTAT1854.7293724E-1110.400341
CTACCGT750.002659908510.1277694
GTAGGAC16800.010.06053453
TCCTACA15050.010.0316172
GTATCAA20300.09.999441
GGACCAT2002.5465852E-119.9692336
GTCCTAC13800.09.9688771
Produced by FastQC (version 0.11.5)