FastQCFastQC Report
Thu 26 May 2016
SRR1512738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728966
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51200.2961307509806439No Hit
GGTATCAACGCAGAGTACTTTTTTT32870.19011362860808137No Hit
TATCAACGCAGAGTACTTTTTTTTT30900.1787195352598027No Hit
GTCCTACAGTGGACATTTCTAAATT25590.14800753745302106No Hit
CTGTAGGACGTGGAATATGGCAAGA25470.14731348100541017No Hit
GTCCTAAAGTGTGTATTTCTCATTT21170.12244312496602015No Hit
CTTTAGGACGTGAAATATGGCGAGG20640.11937770898907207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGTCG400.00528769314.24663713
ATCCCGT1352.7284841E-1112.66477710
GGTCGGT604.0975938E-412.66477611
TAGGACC5650.012.6182314
TCCAACG2150.011.92500218
GCCGGTT1157.0891474E-811.56349111
CGGTTTC1001.9352883E-611.39730913
GGCGAGG6400.011.12793219
CGTGCGC600.005882641311.08167910
TGCCGGT1201.2779856E-711.08167910
TCCCGTT1553.1286618E-1011.03061211
GTAGGAC13850.010.8440863
TGTAGGA15550.010.820032
CCGGTTT1158.8337583E-710.73752712
CCCGTTT1605.456968E-1010.68590512
GGTATCA14300.010.5785021
CCAACGT909.557972E-510.55092719
CCAACGA1553.6870915E-910.41478619
GGACGTG12200.010.3553116
GTTCTAG2151.8189894E-1210.1777651
Produced by FastQC (version 0.11.5)