FastQCFastQC Report
Thu 26 May 2016
SRR1512737_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512737_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720330
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40130.23326919835147908No Hit
GGTATCAACGCAGAGTACTTTTTTT24420.1419495096870949No Hit
GTACATGGGAAGCAGTGGTATCAAC21440.12462725174821111No Hit
TATCAACGCAGAGTACTTTTTTTTT21260.12358094086599664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA5900.011.7429771
CTTATAC1553.1468517E-1011.0283173
CTAAAGT7250.011.0023214
TAGGACC3550.010.967274
TACCGCA700.001496350910.8529477
CTTACAC2050.010.6547293
CTAATAC1701.4006218E-1010.6138543
GTATAGA1854.7293724E-1110.4029381
AAAGTGT7950.010.392216
TCCTAAA7700.010.3599352
GGCGAGG4400.010.35660319
GACGTGG5200.010.2268167
TAATACC1303.862824E-710.2265174
TAGGACG10200.010.147744
GGATAGG951.4442108E-410.1291761
AGGACGT9750.010.1291225
CCTAGAC1701.5679689E-910.0552313
GTAGGAC10300.010.0498073
TTAGGAC6300.09.9488263
TGTAGGA11900.09.8956232
Produced by FastQC (version 0.11.5)