FastQCFastQC Report
Thu 26 May 2016
SRR1512737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720330
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46890.2725639848168665No Hit
GGTATCAACGCAGAGTACTTTTTTT30110.17502455924154087No Hit
TATCAACGCAGAGTACTTTTTTTTT28270.1643289368900153No Hit
GTACATGGGAAGCAGTGGTATCAAC23550.13689234042305837No Hit
GTCCTACAGTGGACATTTCTAAATT19970.1160823795434597No Hit
CTGTAGGACGTGGAATATGGCAAGA17290.10050397307493329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA550.00308404712.08284519
TAGGACC3700.011.563494
TTAGCCC803.7427698E-410.6962284
GTAGGAC10300.010.52314953
GGCGAGG4250.010.49887519
TGGCGAG9300.010.41240518
TGTACCG1105.984568E-610.3724025
GTCCTAC9600.010.311481
TGACCCG750.002629308510.1419045
ACACCGT750.002650978710.1318756
TAGGACG10850.010.0773484
AGGACGT10300.010.0619015
GGACGTG10250.010.0083166
TGTAGGA11650.09.9583632
TTAGGAC6900.09.9211393
CTGTAGG10900.09.6929361
GTCTTAA1901.0149961E-99.518291
TACATCG800.00448447239.5080365
AATACTG2401.8189894E-129.5080355
TAGCACC1701.6420017E-89.5077594
Produced by FastQC (version 0.11.5)