FastQCFastQC Report
Thu 26 May 2016
SRR1512736_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512736_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856900
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47620.2564489202434165No Hit
GTCCTACAGTGGACATTTCTAAATT30310.16322903764338415No Hit
GGTATCAACGCAGAGTACTTTTTTT28670.15439711346868437No Hit
CTGTAGGACGTGGAATATGGCAAGA28450.1532123431525661No Hit
GTCCTAAAGTGTGTATTTCTCATTT28430.1531046367601917No Hit
TATCAACGCAGAGTACTTTTTTTTT27570.14847326188809307No Hit
CTTTAGGACGTGAAATATGGCGAGG25770.13877968657439818No Hit
GTACATGGGAAGCAGTGGTATCAAC23270.12531638752759977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG500.001505645813.29305654
TAGGACC5600.012.8861264
TCCAACG1302.6448106E-911.68398718
GGACCTG11150.011.15500456
GAAACGG600.00590758311.07544618
GGCGAGG7450.010.95893619
AGGACCT12300.010.6529715
AATCCCG1252.2615859E-710.63271619
GTCTAAG1552.9722287E-910.5523331
GTCCTAC15900.010.4078871
TGGCGAG17800.010.39989818
TGTAGGA17350.010.289692
AATGTCC5150.010.2881788
GTGTAGG3850.010.2460011
GGACGTG17200.010.1569356
AGGACGT17750.010.1102125
GCTATAC1701.5697879E-910.0546623
GTATTAG1252.185192E-610.0060941
CTAGGAC2002.5465852E-119.9708743
GTAGGAC17250.09.9639913
Produced by FastQC (version 0.11.5)