FastQCFastQC Report
Thu 26 May 2016
SRR1512736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856900
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56920.3065323926975066No Hit
GGTATCAACGCAGAGTACTTTTTTT36490.19651031288706985No Hit
TATCAACGCAGAGTACTTTTTTTTT36090.1943561850395821No Hit
CTGTAGGACGTGGAATATGGCAAGA33260.1791157305186063No Hit
GTCCTACAGTGGACATTTCTAAATT32530.17518444719694115No Hit
GTCCTAAAGTGTGTATTTCTCATTT31050.16721417416123646No Hit
CTTTAGGACGTGAAATATGGCGAGG28520.15358931552587646No Hit
GTACATGGGAAGCAGTGGTATCAAC26500.14271096989606333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAT1207.421477E-1012.66472712
ACCACGC853.951236E-612.2922358
CGCTCAT701.0926815E-412.21241510
CGAAATC1302.6284397E-911.68894213
GGCGAGG7800.010.95543219
TGGCGAG17200.010.76434818
TGTAGGA17200.010.4480532
GACGTGG7550.010.4421097
TAGACAG2650.010.4041665
GGACGTG17250.010.2419096
ATAGGAC2053.6379788E-1210.2029083
TAGGACC6250.010.1917774
GATTAGA1502.3797838E-810.1509281
CCATGCG750.00265135310.1317819
GTAGGAC16800.010.0731633
AGGACGT17850.010.0665055
TGTCGAG856.628097E-410.0532218
TAGGACA4750.010.0076374
TAGGACG18700.09.9139824
CGTGGAA8000.09.8547419
Produced by FastQC (version 0.11.5)