FastQCFastQC Report
Thu 26 May 2016
SRR1512735_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512735_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585872
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT45590.287475912305659No Hit
GTCCTAAAGTGTGTATTTCTCATTT41290.2603614919741316No Hit
CTGTAGGACGTGGAATATGGCAAGA40520.2555061190310441No Hit
GTATCAACGCAGAGTACTTTTTTTT37800.23835467175156635No Hit
CTTTAGGACGTGAAATATGGCGAGG35800.22574331345783263No Hit
GTCCTACAGTGTGCATTTCTCATTT25710.16211901086594632No Hit
GGTATCAACGCAGAGTACTTTTTTT23350.14723760807934055No Hit
TATCAACGCAGAGTACTTTTTTTTT21150.13336511395623354No Hit
GTACATGGGAAGCAGTGGTATCAAC19670.12403270881887062No Hit
CTGAAGGACCTGGAATATGGCGAGA17970.11331305426919701No Hit
CTGTAGGACCTGGAATATGGCGAGA17050.10751182945407953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG402.769777E-416.61825
CGCTTGG400.00531245414.23649710
TAGGACC7000.013.5658794
AAGGCGT500.001504193313.2945616
TCCAACG1900.012.98973918
CCAACGT1052.0110747E-812.65867619
GCCGGTT1650.012.65546611
GGACCTA802.8784447E-511.8701446
GGCGAGG12100.011.84789419
GACAGCG658.051109E-411.6875277
TGCCGGT1800.011.60010910
AGGACCT13850.011.5187535
GTCTTAC1709.094947E-1211.31981
GCGCAGA600.005343772511.225471
GGACCTG13400.011.1260456
TGTAGGA27350.011.0759082
GTGTAGG4000.011.06510451
CGGTTTC1900.010.99619913
CTAGGAC2600.010.9570563
AATGTCC6150.010.9262668
Produced by FastQC (version 0.11.5)