FastQCFastQC Report
Thu 26 May 2016
SRR1512735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512735_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585872
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46910.2957994087795232No Hit
GTATCAACGCAGAGTACTTTTTTTT45830.288989275300907No Hit
CTGTAGGACGTGGAATATGGCAAGA44510.2806657788270428No Hit
GTCCTAAAGTGTGTATTTCTCATTT42340.26698245507834173No Hit
CTTTAGGACGTGAAATATGGCGAGG39990.2521641090832047No Hit
GGTATCAACGCAGAGTACTTTTTTT29320.18488251258613558No Hit
TATCAACGCAGAGTACTTTTTTTTT28560.1800901964345168No Hit
GTCCTACAGTGTGCATTTCTCATTT25340.15978590958160557No Hit
GTACATGGGAAGCAGTGGTATCAAC23050.14534590433528052No Hit
CTGAAGGACCTGGAATATGGCGAGA21090.13298677320742153No Hit
CTGTAGGACCTGGAATATGGCGAGA19080.12031235812221919No Hit
TTTCTAAATTTTCCACCTTTTTCAG18090.11406973576682103No Hit
ATTTAGAAATGTCCACTGTAGGACG16400.10341313800861608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTT250.00603855418.996510
CCAACGA1004.1836756E-1115.19240719
TCCAACG1450.013.09690318
ACCGTGC701.0928053E-412.2120348
TAGGACC8100.012.2060644
CCGCTCA550.003072729612.0886819
AAGGCGT802.8718552E-511.8728116
GGCGAGG11550.011.42718919
GGACCAT1604.5474735E-1111.2791716
ACCTTTT6500.011.10144215
CACCTTT6850.011.08969714
GAACAGT3600.011.0812916
GCGTGCG600.005883813411.081299
CCACCTT6100.011.053596513
AATCCCG2500.011.01449519
GCCGGTT951.362912E-510.99797311
GTCCTAT1657.8216544E-1110.9570791
CTAGACT1658.0035534E-1110.9470474
AGGACGT25900.010.8283755
GACGTGA14050.010.8165117
Produced by FastQC (version 0.11.5)