FastQCFastQC Report
Thu 26 May 2016
SRR1512734_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512734_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717145
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46900.2731277789586785No Hit
GTCCTACAGTGGACATTTCTAAATT30270.17628097801874623No Hit
GGTATCAACGCAGAGTACTTTTTTT27930.1626537071709145No Hit
GTCCTAAAGTGTGTATTTCTCATTT27170.1582277559553794No Hit
TATCAACGCAGAGTACTTTTTTTTT26270.1529864979369826No Hit
CTGTAGGACGTGGAATATGGCAAGA26190.15252060833534734No Hit
CTTTAGGACGTGAAATATGGCGAGG25350.147628767518177No Hit
GTCCTACAGTGTGCATTTCTCATTT17690.10301983816159964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5600.013.9048564
AGGACCT11000.011.3088725
TCCAACG1354.791218E-911.24957218
TAGGTCG600.005894509611.0786665
GGTATCA10800.011.0531391
GGACCTA1451.4526449E-810.4783446
GTCCTAA9400.010.4462411
GGACCTG10600.010.3918496
ATCCCGT1106.128972E-610.351686510
GTATTAA2800.010.3145661
TCTATAC1752.3646862E-1010.3105473
CCAACGA1303.8724465E-710.22466219
GTCCTAC16400.010.2139361
GTCTTAC1803.092282E-1010.1617571
TGTAGGA18750.010.0790122
ATACCGT856.619228E-410.0545876
CCTACAC2850.09.9963753
CGTTTCC1252.5650916E-69.88134214
GTTCTAG2053.092282E-119.8617311
CCGTTTC1356.465616E-79.84914613
Produced by FastQC (version 0.11.5)