FastQCFastQC Report
Thu 26 May 2016
SRR1512734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512734_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717145
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53530.3117383796942017No Hit
GGTATCAACGCAGAGTACTTTTTTT34690.20202137850909505No Hit
TATCAACGCAGAGTACTTTTTTTTT32980.1920629882741411No Hit
GTCCTACAGTGGACATTTCTAAATT32610.18990824886657795No Hit
CTGTAGGACGTGGAATATGGCAAGA31020.18064869303407693No Hit
GTCCTAAAGTGTGTATTTCTCATTT29800.173543876609139No Hit
CTTTAGGACGTGAAATATGGCGAGG27280.15886835415762793No Hit
GTCCTACAGTGTGCATTTCTCATTT18430.1073293169767259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT555.9182275E-717.270586
ACCGCGG350.002172971116.2827438
GACCGCT508.724018E-515.1981137
GCCGGTT903.648347E-813.71971911
TGGGCGA500.001501721613.29757310
TGGACCG957.3107E-813.0105155
CCGGTTT957.408926E-812.99724912
TAGGACC6300.012.8274544
GGCGAGG6650.011.85014519
GACCGTG752.076449E-411.3985847
GGACCGC752.076449E-411.3985846
TGTAGGA16950.010.9947782
CTAATAC1053.4340737E-610.8656083
AATCCCG1752.0008883E-1110.85073619
CTAGGGG909.440583E-510.5637863
AGGACCT11600.010.4912385
GTAGGAC17050.010.4832413
AAGTCGT1451.4459147E-810.4814567
GACGTGG8600.010.3824327
GATATAC4250.010.3014851
Produced by FastQC (version 0.11.5)