FastQCFastQC Report
Thu 26 May 2016
SRR1512733_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512733_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589455
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45040.28336756938699115No Hit
GGTATCAACGCAGAGTACTTTTTTT25700.1616906423900016No Hit
TATCAACGCAGAGTACTTTTTTTTT23940.1506176645453945No Hit
GTCCTACAGTGGACATTTCTAAATT23920.15049183525170576No Hit
GTCCTAAAGTGTGTATTTCTCATTT21700.13652478365225817No Hit
CTGTAGGACGTGGAATATGGCAAGA20860.13123995331733204No Hit
CTTTAGGACGTGAAATATGGCGAGG17870.11242847391086883No Hit
GTACATGGGAAGCAGTGGTATCAAC16300.10255087435630451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG400.00529253514.2445634
TCCAACG801.3009958E-714.23870518
TAGGACC4950.013.6210644
CGTGCAT751.4916934E-512.65582510
CCGGTCC802.9274304E-511.850599
GTAGGAC13600.011.8010173
TGTAGGA14450.011.7640492
TATGTCG600.00586611511.085772516
CTGTAGG14100.010.9107171
CCCGGTC700.001540192410.8153448
GTAGAAC4250.010.7256573
CTTACAC1605.475158E-1010.6837613
TAAGCCC803.784067E-410.6834234
GTCCTAC12500.010.6150371
AGGACGT12900.010.600275
TAGGACG12950.010.4863464
GGACGTG12800.010.4601896
TATACTG2200.010.3593555
GTGTAGG2700.010.3272961
GTACTAA1408.119969E-810.301861