FastQCFastQC Report
Thu 26 May 2016
SRR1512733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589455
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55230.34747759452139254No Hit
GGTATCAACGCAGAGTACTTTTTTT33440.21038657904753516No Hit
TATCAACGCAGAGTACTTTTTTTTT32280.20308848001358956No Hit
CTGTAGGACGTGGAATATGGCAAGA24550.15445545800290036No Hit
GTCCTACAGTGGACATTTCTAAATT24170.15206470142281472No Hit
GTCCTAAAGTGTGTATTTCTCATTT22570.14199835792771737No Hit
CTTTAGGACGTGAAATATGGCGAGG20270.1275279891535149No Hit
GTACATGGGAAGCAGTGGTATCAAC18190.11444174260988829No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16110.10135549606626171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA704.4661283E-714.91969619
TAGGACC4800.013.666624
CTATGCG500.001502027513.2971149
AACAGCG550.003073488812.0882857
TCCAACG1302.6393536E-911.68535718
GGTTTAA1001.883147E-611.4230531
GTCTTAT1408.089046E-910.8790981
AGAACCG803.742232E-410.696295
GTTAGTC803.7444118E-410.6956163
CTAGACA1701.364242E-1010.62571054
TTAGGAC7250.010.6218543
GTTTAGG1451.39807526E-810.5039571
AGGACGT11950.010.4227925
GTATTAG1409.402538E-810.1991551
ATTATAC1409.577525E-810.1863013
AAATGTC4200.010.1763627
GGACGTG12250.010.1579396
TAGACAG2550.010.0670985
GTCCTAA7250.09.9787591
GTACTAG1054.01425E-59.97250651
Produced by FastQC (version 0.11.5)