FastQCFastQC Report
Thu 26 May 2016
SRR1512732_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512732_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902627
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49000.2575386557638465No Hit
GGTATCAACGCAGAGTACTTTTTTT28830.15152733562595297No Hit
TATCAACGCAGAGTACTTTTTTTTT27470.14437932395577271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTT550.003079056312.085565
ATCCGAG700.001491245810.85750616
GTAGAAC3150.010.85323
GGGTCGT700.00149816910.8514797
TAGGACC2750.010.7046374
GTATTAG1451.1785232E-810.62017251
CTAAGAC1701.4006218E-1010.6137913
ATAATAC1951.4551915E-1110.2270543
GGTATCA13500.010.1235811
GGTCTAA1158.792327E-610.042991
CTTATTC1054.1219042E-59.9487673
CTAGGAC2201.8189894E-129.9282113
TAGAACA3650.09.6261314
GTCCTAA3800.09.6245321
GTATAGA2500.09.6245321
GTATAAG1605.426591E-89.6245311
TATACTG3000.09.4957975
TGGACGT800.004532739.4957965
AGTGCGG900.00112368369.4907810
GTGTAGG3050.09.4667521
Produced by FastQC (version 0.11.5)