FastQCFastQC Report
Thu 26 May 2016
SRR1512732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902627
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60610.31855954950707627No Hit
GGTATCAACGCAGAGTACTTTTTTT38440.2020364475012706No Hit
TATCAACGCAGAGTACTTTTTTTTT37250.1957819372898629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT2150.011.05353454
GGTATCA15550.010.8909691
TCTTATA1855.456968E-1210.7929112
CCTATAC1553.6252459E-910.4259793
GTATCAA31350.010.1971451
ACACCGT750.002650835610.1320436
TCGTGTG951.6468119E-49.9984649
TAAAGGT2300.09.9193454
CCTACAC2750.09.6788763
GTCCTAA4800.09.5143011
GTGCTAA1304.1581843E-69.5143011
GTCTTAT1304.1581843E-69.5143011
CGTACAC900.00110729889.506043
TAGGACA3950.09.385714
GTAGGAC7000.09.3702383
ACTACAC2151.05501385E-109.2849693
GTTCTAG1856.524715E-99.2571571
GTCTTAG1651.0410986E-79.2259891
GTATAAA3550.09.1122881
GTCCTAC5650.09.0933141
Produced by FastQC (version 0.11.5)