FastQCFastQC Report
Thu 26 May 2016
SRR1512731_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512731_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96181
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30283.148230939582662No Hit
GGTATCAACGCAGAGTACTTTTTTT24502.54728064794502No Hit
TATCAACGCAGAGTACTTTTTTTTT21252.2093760721972115No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11141.158232915024797No Hit
GTACTGGTTCACTATCGGTCAGTCA11021.145756438381801No Hit
GTACATGGGAAGCAGTGGTATCAAC10171.0573813954939126No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9791.0178725527910917No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7860.8172092201162391No Hit
GGATACCACGTGTCCCGCCCTACTC6180.6425385471142949No Hit
CCCATGTACTCTGCGTTGATACCAC6120.636300308792797No Hit
ATCATTAACTGAATCCATAGGTTAA5920.6155061810544702No Hit
ATACAGGGTGACAGCCCCGTACACA5580.5801561638993149No Hit
GTACATGGGGGTTAAGCGACTAAGC5460.5676796872563188No Hit
GGTATCAACGCAGAGTACATGGGGG5120.5323296701011634No Hit
GGTTAATGAGGCGAACCGGGGGAAC4660.48450317630301204No Hit
GTATTTAGCCTTGGAGGATGGTCCC4640.4824237635291794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4600.47826493798151404No Hit
GAGTACATGGGAAGCAGTGGTATCA4480.465788461338518No Hit
GTGGATGCCCTGGCAGTCAGAGGCG4210.437716388891777No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4170.4335575633441116No Hit
GTGGTATCAACGCAGAGTACTTTTT4100.42627961863569724No Hit
GTATCAACGCAGAGTACATGGGGGT3820.3971678398020399No Hit
GCCTTGGAGGATGGTCCCCCCATAT3680.38261195038521123No Hit
GTTAATGATAGTGTGTCGAAACACA3590.3732545929029642No Hit
CCCATATTCAGACAGGATACCACGT3500.36389723542071717No Hit
ACTTAGATGTTTCAGTTCCCCCGGT3470.3607781162599682No Hit
TCTAAGTACCCCGAGGAAAAGAAAT3460.35973840987305183No Hit
CTATCGGTCAGTCAGGAGTATTTAG3370.35038105239080486No Hit
GTCAGGAGTATTTAGCCTTGGAGGA3350.3483016396169722No Hit
GTTCACTATCGGTCAGTCAGGAGTA3250.33790457574780886No Hit
GGTATCAACGCAGAGTACATGGGAA3210.33374575020014347No Hit
GTGGTATCAACGCAGAGTACATGGG3180.3306266310393945No Hit
AGCGTACACGGTGGATGCCCTGGCA3160.32854721826556177No Hit
GCCCAGAGCCTGAATCAGTGTGTGT3100.3223089799440638No Hit
ACCCTGTATCGCGCGCCTTTCCAGA2940.3056736777534025No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT2930.3046339713664861No Hit
GTGCTAATCTGCGATAAGCGTCGGT2850.2963163202711554No Hit
TATTCAGACAGGATACCACGTGTCC2850.2963163202711554No Hit
CGGTACTGGTTCACTATCGGTCAGT2840.2952766138842391No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG2840.2952766138842391No Hit
ACGCAGAGTACATGGGGGTTAAGCG2730.2838398436281594No Hit
GTTATAACCGGCGATTTCCGAATGG2710.2817604308543267No Hit
GGTTCACTATCGGTCAGTCAGGAGT2600.2703236605982471No Hit
TATCAACGCAGAGTACATGGGAAGC2590.2692839542113307No Hit
TCACGGTACTGGTTCACTATCGGTC2520.26200600950291636No Hit
CATCTAAGTACCCCGAGGAAAAGAA2500.2599265967290837No Hit
GTATCAACGCAGAGTACATGGGAAG2480.2578471839552511No Hit
GCCTGAATCAGTGTGTGTGTTAGTG2470.2568074775683347No Hit
GATATGAACCGTTATAACCGGCGAT2460.25576777118141836No Hit
ATCAACGCAGAGTACTTTTTTTTTT2420.251608945633753No Hit
GGAGTATTTAGCCTTGGAGGATGGT2400.24952953285992036No Hit
GTACTTAGATGTTTCAGTTCCCCCG2400.24952953285992036No Hit
GAGTACATGGGGGTTAAGCGACTAA2350.2443310009253387No Hit
GGTATCAACGCAGAGTACATGGGGA2220.23081481789542632No Hit
ATCCATAGGTTAATGAGGCGAACCG2180.226655992347761No Hit
GAATCAGTGTGTGTGTTAGTGGAAG2170.22561628596084465No Hit
GCGTTGATACCACTGCTTCCCATGT2100.2183383412524303No Hit
TATCAACGCAGAGTACATGGGGGTT2080.21625892847859765No Hit
ACGCAGAGTACATGGGAAGCAGTGG2080.21625892847859765No Hit
GGTGATATGAACCGTTATAACCGGC2000.20794127738326695No Hit
GCCCTACTCATCGAGCTCACAGCAT1940.20170303906176895No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG1930.20066333267485262No Hit
GTCCCGCCCTACTCATCGAGCTCAC1890.19650450712718728No Hit
CATGTACTCTGCGTTGATACCACTG1890.19650450712718728No Hit
CACTATCATTAACTGAATCCATAGG1840.19130597519260562No Hit
CCCCAGTAGCGGCGAGCGAACGGGG1820.18922656241877295No Hit
CACACACACTGATTCAGGCTCTGGG1820.18922656241877295No Hit
TCATTAACCTATGGATTCAGTTAAT1810.1881868560318566No Hit
GGGTGACAGCCCCGTACACAAAAAT1800.18714714964494025No Hit
GGGTACTTAGATGTTTCAGTTCCCC1800.18714714964494025No Hit
CTATCATTAACTGAATCCATAGGTT1700.17675008577577694No Hit
GCGTACACGGTGGATGCCCTGGCAG1680.17467067300194425No Hit
TCGGTAAGGTGATATGAACCGTTAT1650.17155155384119525No Hit
AGCTCACAGCATGTGCATTTTTGTG1610.1673927282935299No Hit
TTCCAGACGCTTCCACTAACACACA1540.16011478358511558No Hit
CCTATGGATTCAGTTAATGATAGTG1530.15907507719819924No Hit
TTTGTGTACGGGGCTGTCACCCTGT1520.15803537081128288No Hit
GGGAAACCCAGTGTGTTTCGACACA1520.15803537081128288No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA1510.15699566442436655No Hit
GGGTTTCCCCATTCGGAAATCGCCG1470.15283683887670124No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1450.15075742610286855No Hit
GTCCCCCCATATTCAGACAGGATAC1430.14867801332903588No Hit
TAAGTACCCCGAGGAAAAGAAATCA1430.14867801332903588No Hit
ACTCTGCGTTGATACCACTGCTTCC1390.14451918778137054No Hit
GGAGGATGGTCCCCCCATATTCAGA1350.14036036223370518No Hit
CCGTTATAACCGGCGATTTCCGAAT1340.13932065584678885No Hit
GTCAGAGGCGATGAAGGACGTGCTA1330.13828094945987252No Hit
AAGTACCCCGAGGAAAAGAAATCAA1300.13516183029912354No Hit
GTGTTTCGACACACTATCATTAACT1260.13100300475145818No Hit
GTGCATTTTTGTGTACGGGGCTGTC1250.12996329836454185No Hit
GTGGTATCCTGTCTGAATATGGGGG1250.12996329836454185No Hit
GAATATGGGGGGACCATCCTCCAAG1230.12788388559070918No Hit
GGTATCAACGCAGAGTACATGGGCA1230.12788388559070918No Hit
GATCAACGCAGAGTACTTTTTTTTT1220.12684417920379285No Hit
GGGAAGCAGTGGTATCAACGCAGAG1220.12684417920379285No Hit
ATGATAGTGTGTCGAAACACACTGG1220.12684417920379285No Hit
NTATCAACGCAGAGTACTTTTTTTT1220.12684417920379285No Hit
GTACATGGGCATCTAAGTACCCCGA1220.12684417920379285No Hit
ATATCAACGCAGAGTACTTTTTTTT1210.1258044728168765No Hit
AGTCAGGAGTATTTAGCCTTGGAGG1200.12476476642996018No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1200.12476476642996018No Hit
TCCCCCAGTAGCGGCGAGCGAACGG1200.12476476642996018No Hit
GGGGAAACCCAGTGTGTTTCGACAC1190.12372506004304384No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA1180.12268535365612752No Hit
GATTCAGGCTCTGGGCTGCTCCCCG1170.12164564726921116No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC1160.12060594088229484No Hit
GTACTCTGCGTTGATACCACTGCTT1160.12060594088229484No Hit
ATTTAGCCTTGGAGGATGGTCCCCC1150.11956623449537851No Hit
AACGCAGAGTACTTTTTTTTTTTTT1150.11956623449537851No Hit
CAGTAGCGGCGAGCGAACGGGGAGC1140.11852652810846218No Hit
GGATGGTCCCCCCATATTCAGACAG1110.11540740894771316No Hit
GCATGTGCATTTTTGTGTACGGGGC1100.11436770256079683No Hit
CCCTGGCAGTCAGAGGCGATGAAGG1080.11228828978696416No Hit
CATTAACTGAATCCATAGGTTAATG1080.11228828978696416No Hit
GCAGTGGTATCAACGCAGAGTACAT1080.11228828978696416No Hit
GTACGGGGCTGTCACCCTGTATCGC1080.11228828978696416No Hit
TAATGATAGTGTGTCGAAACACACT1070.11124858340004784No Hit
AGTATCAACGCAGAGTACTTTTTTT1060.11020887701313148No Hit
GGTATCAACGCAGAGTACATGGGAT1040.10812946423929883No Hit
GTATCGCGCGCCTTTCCAGACGCTT1030.10708975785238249No Hit
GCTTCCCATGTACTCTGCGTTGATA1030.10708975785238249No Hit
GTATCAACGCAGAGTACATGGGGAA1030.10708975785238249No Hit
GGGCTGTCACCCTGTATCGCGCGCC1030.10708975785238249No Hit
GCAGAGTACATGGGAAGCAGTGGTA1030.10708975785238249No Hit
CAGTGGTATCAACGCAGAGTACATG1020.10605005146546616No Hit
GAGCAGCCCAGAGCCTGAATCAGTG1020.10605005146546616No Hit
NGTATCAACGCAGAGTACTTTTTTT990.10293093230471714No Hit
CATGGGAAGCAGTGGTATCAACGCA980.10189122591780081No Hit
GAGTAGGGCGGGACACGTGGTATCC970.10085151953088448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAA400.00528029614.2300062
GAACCAG400.00528029614.2300066
CCCCCGG707.275694E-613.53823218
TGAACCA500.001497447813.2813385
ACCGGGG801.9750169E-613.04417215
AGGGTGA751.4655745E-512.6488935
CAGGGTG751.4655745E-512.6488934
CGGGGGA905.353486E-712.64228517
CCCCGGT751.4814448E-512.63568319
CCGGGGG853.8959897E-612.27686716
ACAGGGT853.8959897E-612.2768673
TCCCCCG802.8569108E-511.85214217
GGGGAAC1104.938156E-711.1998119
GGGTGAC855.2776202E-511.1607896
AGCGACT1201.24488E-711.06778215
CCGCCCT1252.1870437E-710.62507115
GGTATCA8250.010.6170511
GGTGACA909.4353134E-510.5407457
GTGACAG909.688977E-510.5132678
CGCCCTA1303.7511745E-710.216413516
Produced by FastQC (version 0.11.5)