FastQCFastQC Report
Thu 26 May 2016
SRR1512731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96181
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32383.3665692808350927No Hit
GGTATCAACGCAGAGTACTTTTTTT26182.721951320946965No Hit
TATCAACGCAGAGTACTTTTTTTTT23292.4214761751281437No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12211.2694814984248448No Hit
GTACTGGTTCACTATCGGTCAGTCA11321.176947629989291No Hit
GTACATGGGAAGCAGTGGTATCAAC10171.0573813954939126No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9751.0137137272434265No Hit
GGATACCACGTGTCCCGCCCTACTC8020.8338445223069005No Hit
CCCATGTACTCTGCGTTGATACCAC7650.7953753859909962No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7510.7808194965741674No Hit
ATCATTAACTGAATCCATAGGTTAA7290.7579459560620081No Hit
ATACAGGGTGACAGCCCCGTACACA6230.6477370790488766No Hit
GGTTAATGAGGCGAACCGGGGGAAC6170.6414988407273786No Hit
GGTATCAACGCAGAGTACATGGGGG5170.5375282020357451No Hit
GTACATGGGGGTTAAGCGACTAAGC4960.5156943679105022No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4900.5094561295890041No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4890.5084164232020878No Hit
GTGGATGCCCTGGCAGTCAGAGGCG4800.4990590657198407No Hit
GTATTTAGCCTTGGAGGATGGTCCC4760.4949002401721754No Hit
GAGTACATGGGAAGCAGTGGTATCA4720.4907414146245101No Hit
CCCATATTCAGACAGGATACCACGT4490.46682816772543434No Hit
AGCGTACACGGTGGATGCCCTGGCA4230.4397958016656096No Hit
GTGGTATCAACGCAGAGTACTTTTT4220.4387560952786933No Hit
GTTAATGATAGTGTGTCGAAACACA3990.4148428483796176No Hit
TATTCAGACAGGATACCACGTGTCC3970.412763435605785No Hit
GCCTTGGAGGATGGTCCCCCCATAT3950.41068402283195227No Hit
TCTAAGTACCCCGAGGAAAAGAAAT3870.4023663717366216No Hit
CGGTACTGGTTCACTATCGGTCAGT3830.3982075461889562No Hit
CTATCGGTCAGTCAGGAGTATTTAG3710.38573106954596026No Hit
GTATCAACGCAGAGTACATGGGGGT3670.38157224399829487No Hit
GTGGTATCAACGCAGAGTACATGGG3650.3794928312244622No Hit
GTTCACTATCGGTCAGTCAGGAGTA3610.37533400567679687No Hit
GTCAGGAGTATTTAGCCTTGGAGGA3570.37117518012913153No Hit
TCACGGTACTGGTTCACTATCGGTC3560.3701354737422152No Hit
ACTTAGATGTTTCAGTTCCCCCGGT3520.3659766481945499No Hit
ATCAACGCAGAGTACTTTTTTTTTT3390.3524604651646375No Hit
ACCCTGTATCGCGCGCCTTTCCAGA3350.3483016396169722No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT3330.34622222684313947No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG3220.3347854565870598No Hit
TATCAACGCAGAGTACATGGGAAGC3180.3306266310393945No Hit
GGTATCAACGCAGAGTACATGGGAA3140.3264678054917291No Hit
GGTTCACTATCGGTCAGTCAGGAGT3110.32334868633098013No Hit
CATCTAAGTACCCCGAGGAAAAGAA3080.32022956717023116No Hit
GTTATAACCGGCGATTTCCGAATGG3040.31607074162256577No Hit
CATGTACTCTGCGTTGATACCACTG3030.31503103523564946No Hit
GCCTGAATCAGTGTGTGTGTTAGTG3010.3129516224618168No Hit
GCCCAGAGCCTGAATCAGTGTGTGT3010.3129516224618168No Hit
ACGCAGAGTACATGGGGGTTAAGCG2800.29111778833657376No Hit
GGTATCAACGCAGAGTACATGGGGA2770.2879986691758248No Hit
GATATGAACCGTTATAACCGGCGAT2760.2869589627889084No Hit
GTGCTAATCTGCGATAAGCGTCGGT2700.28072072446741037No Hit
GCGTTGATACCACTGCTTCCCATGT2680.2786413116935777No Hit
GTACTTAGATGTTTCAGTTCCCCCG2630.27344277975899606No Hit
ACGCAGAGTACATGGGAAGCAGTGG2630.27344277975899606No Hit
TCATTAACCTATGGATTCAGTTAAT2600.2703236605982471No Hit
GTATCAACGCAGAGTACATGGGAAG2570.26720454143749806No Hit
GAATCAGTGTGTGTGTTAGTGGAAG2500.2599265967290837No Hit
CACTATCATTAACTGAATCCATAGG2470.2568074775683347No Hit
TATCAACGCAGAGTACATGGGGGTT2460.25576777118141836No Hit
GGAGTATTTAGCCTTGGAGGATGGT2350.2443310009253387No Hit
GAGTACATGGGGGTTAAGCGACTAA2280.23705305621692435No Hit
GGTGATATGAACCGTTATAACCGGC2280.23705305621692435No Hit
CCCCAGTAGCGGCGAGCGAACGGGG2200.22873540512159365No Hit
CACACACACTGATTCAGGCTCTGGG2130.22145746041317932No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2120.22041775402626296No Hit
TCGGTAAGGTGATATGAACCGTTAT2100.2183383412524303No Hit
ATCCATAGGTTAATGAGGCGAACCG1990.20690157099635065No Hit
GGGTGACAGCCCCGTACACAAAAAT1980.2058618646094343No Hit
GCCCTACTCATCGAGCTCACAGCAT1960.20378245183560162No Hit
CCTATGGATTCAGTTAATGATAGTG1890.19650450712718728No Hit
GTGGTATCCTGTCTGAATATGGGGG1880.19546480074027092No Hit
CTATCATTAACTGAATCCATAGGTT1860.19338538796643828No Hit
GGGTACTTAGATGTTTCAGTTCCCC1850.19234568157952195No Hit
GGGAAACCCAGTGTGTTTCGACACA1810.1881868560318566No Hit
CCGTTATAACCGGCGATTTCCGAAT1780.1850677368711076No Hit
CAGTAGCGGCGAGCGAACGGGGAGC1780.1850677368711076No Hit
ACTCTGCGTTGATACCACTGCTTCC1760.18298832409727495No Hit
AGCTCACAGCATGTGCATTTTTGTG1730.17986920493652592No Hit
GTCCCGCCCTACTCATCGAGCTCAC1710.17778979216269325No Hit
TTTGTGTACGGGGCTGTCACCCTGT1670.17363096661502792No Hit
TAAGTACCCCGAGGAAAAGAAATCA1660.17259126022811158No Hit
GATTCAGGCTCTGGGCTGCTCCCCG1660.17259126022811158No Hit
ATTTAGCCTTGGAGGATGGTCCCCC1610.1673927282935299No Hit
GGGTTTCCCCATTCGGAAATCGCCG1600.16635302190661358No Hit
GCGTACACGGTGGATGCCCTGGCAG1570.16323390274586455No Hit
ATGATAGTGTGTCGAAACACACTGG1560.16219419635894822No Hit
CATTAACTGAATCCATAGGTTAATG1550.1611544899720319No Hit
AGTCAGGAGTATTTAGCCTTGGAGG1520.15803537081128288No Hit
GGGAAGCAGTGGTATCAACGCAGAG1520.15803537081128288No Hit
TCCCCCAGTAGCGGCGAGCGAACGG1500.15595595803745022No Hit
GGTATCAACGCAGAGTACATGGGCA1480.15387654526361755No Hit
GTCCCCCCATATTCAGACAGGATAC1480.15387654526361755No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1420.14763830694211955No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA1410.1465986005552032No Hit
GAATATGGGGGGACCATCCTCCAAG1410.1465986005552032No Hit
GGAGGATGGTCCCCCCATATTCAGA1410.1465986005552032No Hit
GAGTAGGGCGGGACACGTGGTATCC1390.14451918778137054No Hit
TTCCAGACGCTTCCACTAACACACA1390.14451918778137054No Hit
CCCTGGCAGTCAGAGGCGATGAAGG1350.14036036223370518No Hit
CAACGCAGAGTACTTTTTTTTTTTT1350.14036036223370518No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1350.14036036223370518No Hit
GTACATGGGCATCTAAGTACCCCGA1340.13932065584678885No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA1330.13828094945987252No Hit
CTACTGGGGGAATCTCGGTTGATTT1330.13828094945987252No Hit
GCTTCCCATGTACTCTGCGTTGATA1330.13828094945987252No Hit
GTGCATTTTTGTGTACGGGGCTGTC1310.13620153668603988No Hit
TAATGATAGTGTGTCGAAACACACT1290.13412212391220718No Hit
GTCAGAGGCGATGAAGGACGTGCTA1280.13308241752529085No Hit
CGTGGTATCCTGTCTGAATATGGGG1270.1320427111383745No Hit
GTCTGAATATGGGGGGACCATCCTC1230.12788388559070918No Hit
CGGTGGATGCCCTGGCAGTCAGAGG1220.12684417920379285No Hit
CGTCTGGAAAGGCGCGCGATACAGG1210.1258044728168765No Hit
AAGTACCCCGAGGAAAAGAAATCAA1210.1258044728168765No Hit
GTGTTTCGACACACTATCATTAACT1190.12372506004304384No Hit
GTATCGCGCGCCTTTCCAGACGCTT1180.12268535365612752No Hit
CAGTGGTATCAACGCAGAGTACATG1180.12268535365612752No Hit
CATTAACCTATGGATTCAGTTAATG1160.12060594088229484No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC1160.12060594088229484No Hit
GTACGGGGCTGTCACCCTGTATCGC1150.11956623449537851No Hit
GCAGTGGTATCAACGCAGAGTACAT1140.11852652810846218No Hit
AACGCAGAGTACTTTTTTTTTTTTT1130.11748682172154584No Hit
CTACTCATCGAGCTCACAGCATGTG1120.1164471153346295No Hit
CTGTCACCCTGTATCGCGCGCCTTT1120.1164471153346295No Hit
GTATCAACGCAGAGTACATGGGGAA1120.1164471153346295No Hit
CTGTGAGCTCGATGAGTAGGGCGGG1100.11436770256079683No Hit
CCACGTGTCCCGCCCTACTCATCGA1100.11436770256079683No Hit
AGGTTAATGAGGCGAACCGGGGGAA1100.11436770256079683No Hit
TCAACGCAGAGTACTTTTTTTTTTT1090.1133279961738805No Hit
GAGCAGCCCAGAGCCTGAATCAGTG1090.1133279961738805No Hit
CACTGATTCAGGCTCTGGGCTGCTC1070.11124858340004784No Hit
TATTTAGCCTTGGAGGATGGTCCCC1060.11020887701313148No Hit
ATTCAGACAGGATACCACGTGTCCC1060.11020887701313148No Hit
AACACACACACTGATTCAGGCTCTG1060.11020887701313148No Hit
CAGGGTGACAGCCCCGTACACAAAA1050.10916917062621515No Hit
CTAACACACACACTGATTCAGGCTC1050.10916917062621515No Hit
ACCCAGTGTGTTTCGACACACTATC1040.10812946423929883No Hit
CATGGGAAGCAGTGGTATCAACGCA1040.10812946423929883No Hit
GCAGAGTACATGGGAAGCAGTGGTA1030.10708975785238249No Hit
GGGGAAACCCAGTGTGTTTCGACAC1020.10605005146546616No Hit
ATACCACTGCTTCCCATGTACTCTG1020.10605005146546616No Hit
ACATGGGAAGCAGTGGTATCAACGC1010.10501034507854982No Hit
AATCTGCGATAAGCGTCGGTAAGGT1010.10501034507854982No Hit
AAAATGCACATGCTGTGAGCTCGAT1000.10397063869163348No Hit
GATGAAGGACGTGCTAATCTGCGAT1000.10397063869163348No Hit
GACTAAGCGTACACGGTGGATGCCC1000.10397063869163348No Hit
GGGGTTAAGCGACTAAGCGTACACG980.10189122591780081No Hit
AACCCAGTGTGTTTCGACACACTAT970.10085151953088448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGG653.3036886E-614.61098419
GATAAGC400.0052420614.24570913
GGGGCTG751.4492314E-512.66285310
CCGAATG751.4492314E-512.66285318
TTTCCGA802.8100912E-511.87142515
CGGGGCT802.8100912E-511.8714259
TTCCGAA802.8100912E-511.87142516
ATTAACC907.199358E-611.61969953
CCATGTA2250.011.4084332
AATCCAT1850.011.29389612
GAATCCA1850.011.29389611
TGAATCC1900.010.99668910
TACTCTG2450.010.8538747
TTAACTG1951.8189894E-1210.7258775
ACTGAAT1951.8189894E-1210.7147228
TAACTGA1951.8189894E-1210.7147226
GGTATCA7900.010.5956221
TACGGGG909.331538E-510.5523777
TCCGAAT909.331538E-510.55237717
GGGCTGT909.331538E-510.55237711
Produced by FastQC (version 0.11.5)